FRET Microscopy in Yeast

• Published in: Biosensors (Basel) Vol. 9; no. 4; p. 122
• Main Authors: Skruzny, Michal, Pohl, Emma, Abella, Marc
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 11-10-2019; MDPI
• Subjects: acceptor photobleaching; Biochemistry; Biomedical materials; Biosensing Techniques; Biosensors; budding yeast; Cell division; Cyclin-dependent kinases; Energy transfer; Experiments; fission yeast; flim; Fluorescence; Fluorescence microscopy; Fluorescence resonance energy transfer; Fluorescence Resonance Energy Transfer - methods; gfp; In vivo methods and tests; Kinases; Luminescent Proteins - analysis; Luminescent Proteins - chemistry; Medical research; Microscopy; Proteins; ratiometric fret; Review; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins - chemistry; Schizosaccharomyces; Schizosaccharomyces pombe Proteins - chemistry; sensitized emission; Sensors; Yeast; Yeasts; GFP; budding yeast; FLIM; fission yeast; sensitized emission; acceptor photobleaching; ratiometric FRET
• ISSN: 2079-6374; 2079-6374
• DOI: 10.3390/bios9040122

Förster resonance energy transfer (FRET) microscopy is a powerful fluorescence microscopy method to study the nanoscale organization of multiprotein assemblies in vivo. Moreover, many biochemical and biophysical processes can be followed by employing sophisticated FRET biosensors directly in living cells. Here, we summarize existing FRET experiments and biosensors applied in yeasts
and
, two important models of fundamental biomedical research and efficient platforms for analyses of bioactive molecules. We aim to provide a practical guide on suitable FRET techniques, fluorescent proteins, and experimental setups available for successful FRET experiments in yeasts.