Progesterone treatment following traumatic brain injury in the 11-day-old rat attenuates cognitive deficits and neuronal hyperexcitability in adolescence

• Published in: Experimental neurology Vol. 330; p. 113329
• Main Authors: Lengel, Dana, Huh, Jimmy W., Barson, Jessica R., Raghupathi, Ramesh
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01-08-2020
• Subjects: Adolescence; Animals; Animals, Newborn; Brain Injuries, Traumatic - complications; Brain Injuries, Traumatic - physiopathology; Cognition; Cognitive Dysfunction - etiology; Cognitive Dysfunction - physiopathology; E/I balance; Excitatory Postsynaptic Potentials - drug effects; Female; Male; Neuronal excitability; Neurons - drug effects; Object recognition; Pediatric TBI; Prefrontal cortex; Prefrontal Cortex - drug effects; Prefrontal Cortex - physiopathology; Progesterone - pharmacology; Rats; Rats, Sprague-Dawley; Risk-taking behavior; Pediatric TBI; Adolescence; Risk-taking behavior; Cognition; E/I balance; Prefrontal cortex; Object recognition; Neuronal excitability
• ISSN: 0014-4886; 1090-2430
• DOI: 10.1016/j.expneurol.2020.113329

Traumatic brain injury (TBI) in children younger than 4 years old results in cognitive and psychosocial deficits in adolescence and adulthood. At 4 weeks following closed head injury on postnatal day 11, male and female rats exhibited impairment in novel object recognition memory (NOR) along with an increase in open arm time in the elevated plus maze (EPM), suggestive of risk-taking behaviors. This was accompanied by an increase in intrinsic excitability and frequency of spontaneous excitatory post-synaptic currents (EPSCs), and a decrease in the frequency of spontaneous inhibitory post-synaptic currents in layer 2/3 neurons within the medial prefrontal cortex (PFC), a region that is implicated in both object recognition and risk-taking behaviors. Treatment with progesterone for the first week after brain injury improved NOR memory at the 4-week time point in both sham and brain-injured rats and additionally attenuated the injury-induced increase in the excitability of neurons and the frequency of spontaneous EPSCs. The effect of progesterone on cellular excitability changes after injury may be related to its ability to decrease the mRNA expression of the β3 subunit of the voltage-gated sodium channel and increase the expression of the neuronal excitatory amino acid transporter 3 in the medial PFC in sham- and brain-injured animals and also increase glutamic acid decarboxylase mRNA expression in sham- but not brain-injured animals. Progesterone treatment did not affect injury-induced changes in the EPM test. These results demonstrate that administration of progesterone immediately after TBI in 11-day-old rats reduces cognitive deficits in adolescence, which may be mediated by progesterone-mediated regulation of excitatory signaling mechanisms within the medial PFC.
•TBI in 11-day-old rats results in cognitive deficits in adolescence.•TBI results in increased excitatory drive in medial prefrontal cortex neurons.•Progesterone attenuates both cognitive deficits and neuronal hyperexcitability.