Trends in SPR Cytometry: Advances in Label-Free Detection of Cell Parameters

SPR cytometry entails the measurement of parameters from intact cells using the surface plasmon resonance (SPR) phenomenon. Specific real-time and label-free binding of living cells to sensor surfaces has been made possible through the availability of SPR imaging (SPRi) instruments and researchers h...

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Bibliographic Details
Published in:	Biosensors (Basel) Vol. 8; no. 4; p. 102
Main Authors:	Schasfoort, Richard B M, Abali, Fikri, Stojanovic, Ivan, Vidarsson, Gestur, Terstappen, Leon W M M
Format:	Journal Article
Language:	English
Published:	Switzerland MDPI AG 30-10-2018 MDPI
Subjects:	Antibodies Antigens Apoptosis Biosensors Blood Cytometry Ligands Mammalian cells Morphology Parameters Review Sensors single-cell secretion SPR imaging Surface plasmon resonance cytometry single-cell secretion surface plasmon resonance SPR imaging
Online Access:	Get full text
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Summary:	SPR cytometry entails the measurement of parameters from intact cells using the surface plasmon resonance (SPR) phenomenon. Specific real-time and label-free binding of living cells to sensor surfaces has been made possible through the availability of SPR imaging (SPRi) instruments and researchers have started to explore its potential in the last decade. Here we will discuss the mechanisms of detection and additionally describe the problems and issues of mammalian cells in SPR biosensing, both from our own experience and with information from the literature. Finally, we build on the knowledge and applications that has already materialized in this field to give a forecast of some exciting applications for SPRi cytometry.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-3 content type line 23 ObjectType-Review-1
ISSN:	2079-6374 2079-6374
DOI:	10.3390/bios8040102