Acinetobacter pullorum sp. nov., Isolated from Chicken Meat

• Published in: Journal of microbiology and biotechnology Vol. 30; no. 4; pp. 526 - 532
• Main Authors: Elnar, Arxel G, Kim, Min-Gon, Lee, Ju-Eun, Han, Rae-Hee, Yoon, Sung-Hee, Lee, Gi-Yong, Yang, Soo-Jin, Kim, Geun-Bae
• Format: Journal Article
• Language: English
• Published: Korea (South) 한국미생물생명공학회 28-04-2020; The Korean Society for Microbiology and Biotechnology; 한국미생물·생명공학회
• Subjects: Acinetobacter - classification; Acinetobacter - cytology; Acinetobacter - drug effects; Acinetobacter - physiology; Acinetobacter pullorum sp. nov; Animals; Anti-Bacterial Agents - pharmacology; antimicrobial resistance; Base Composition; chicken meat; Chickens; DNA, Bacterial - genetics; Fatty Acids - chemistry; Genes, Bacterial; Genome, Bacterial; Microbial Sensitivity Tests; Nucleic Acid Hybridization; Phospholipids - chemistry; Phylogeny; Poultry - microbiology; Quinones - chemistry; Republic of Korea; Research article; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; taxonomy; 생물학; Republic of Korea; taxonomy; antimicrobial resistance; chicken meat; Acinetobacter pullorum sp. nov; nov; Acinetobacter pullorum sp
• ISSN: 1017-7825; 1738-8872
• DOI: 10.4014/jmb.2002.02033

A bacterial strain, designated B301T and isolated from raw chicken meat obtained from a local market in Korea, was characterized and identified using a polyphasic taxonomic approach. Cells were gram-negative, non-motile, obligate-aerobic coccobacilli that were catalase-positive and oxidase-negative. The optimum growth conditions were 30°C, pH 7.0, and 0% NaCl in tryptic soy broth. Colonies were round, convex, smooth, and cream-colored on tryptic soy agar. Strain B301 T has a genome size of 3,102,684 bp, with 2,840 protein-coding genes and 102 RNA genes. The 16S rRNA gene analysis revealed that strain B301 T belongs to the genus Acinetobacter and shares highest sequence similarity (97.12%) with A. celticus ANC 4603 T and A. sichuanensis WCHAc060041 T . The average nucleotide identity and digital DNA-DNA hybridization values for closely related species were below the cutoff values for species delineation (95–96% and 70%, respectively). The DNA G+C content of strain B301 T was 37.0%. The major respiratory quinone was Q-9, and the cellular fatty acids were primarily summed feature 3 (C 16:1 ω6c/C 16:1 ω7c), C 16:0 , and C 18:1 ω9c. The major polar lipids were phosphatidylethanolamine, diphosphatidyl-glycerol, phosphatidylglycerol, and phosphatidyl-serine. The antimicrobial resistance profile of strain B301 T revealed the absence of antibiotic-resistance genes. Susceptibility to a wide range of antimicrobials, including imipenem, minocycline, ampicillin, and tetracycline, was also observed. The results of the phenotypic, chemotaxonomic, and phylogenetic analyses indicate that strain B301 T represents a novel species of the genus Acinetobacter, for which the name Acinetobacter pullorum sp. nov. is proposed. The type strain is B301 T (=KACC 21653 T = JCM 33942 T ).