Natural killer cell cytotoxicity, cytokine and neuroendocrine responses to opioid receptor blockade during prolonged restraint in pigs

• Published in: Research in veterinary science Vol. 95; no. 3; pp. 975 - 985
• Main Authors: Ciepielewski, Z.M., Stojek, W., Borman, A., Myślińska, D., Glac, W., Kamyczek, M.
• Format: Journal Article
• Language: English
• Published: England Elsevier India Pvt Ltd 01-12-2013; Elsevier Limited
• Subjects: Acute prolonged stress; Adrenocorticotropic Hormone - blood; Adrenocorticotropic Hormone - physiology; Animals; beta-Endorphin - blood; beta-Endorphin - physiology; Catheters; corticotropin; cortisol; Cytokines - blood; Cytokines - physiology; Cytotoxicity; Endogenous opioid peptides; Experiments; Growth Hormone - blood; Growth Hormone - physiology; Growth hormones; Hogs; Hybridization; Hydrocortisone - blood; Hydrocortisone - physiology; Immune system; interleukin-10; Interleukin-10 - blood; Interleukin-10 - physiology; interleukin-12; Interleukin-12 - blood; Interleukin-12 - physiology; Interleukin-1beta - blood; Interleukin-1beta - physiology; interleukin-6; Interleukin-6 - blood; Interleukin-6 - physiology; Killer Cells, Natural - physiology; Male; Naloxone; Naloxone - pharmacology; Narcotic Antagonists; Narcotics; Natural killer cell activity; Pig; prolactin; Prolactin - blood; Prolactin - physiology; Restraint; Restraint, Physical - physiology; Restraint, Physical - veterinary; Rodents; somatotropin; Stress; Stress, Physiological - physiology; swine; Swine - physiology; tumor necrosis factor-alpha; Tumor Necrosis Factor-alpha - blood; Tumor Necrosis Factor-alpha - physiology; Veterinary medicine; Endogenous opioid peptides; Restraint; Acute prolonged stress; Naloxone; Natural killer cell activity; Pig
• ISSN: 0034-5288; 1532-2661
• DOI: 10.1016/j.rvsc.2013.09.017

This study evaluated porcine natural killer cell cytotoxicity (NKCC), plasma cytokines including interleukin (IL) 1β, IL-6, IL-10, IL-12 and tumor necrosis factor-α and plasma stress-related hormones including prolactin (PRL), growth hormone (GH), β-endorphin (BEND), ACTH and cortisol (COR) during a 4h restraint and recovery phase after saline or naloxone (1mg/kg BW) administration. The restraint preceded with saline altered NKCC and IL-12 concentration (an early from15 to 60min increase followed by a decrease) and increased other measured cytokines and hormones concentrations. Naloxone pretreatment blocked the suppressive effects of the restraint on NKCC and IL-12 and altered IL-10, IL-6, TNF-α, PRL and ACTH concentrations. Furthermore, in naloxone-injected pigs, a positive correlation was found between NKCC and all measured cytokines (with the exception of IL-6) and BEND, ACTH and COR. Results suggest that naloxone-sensitive opioid pathways could influence the mechanisms underlying the immune system (including NKCC) response during stress.