Draft genome sequence of Lactobacillus delbrueckii subsp. bulgaricus LBP UFSC 2230: a tool for preliminary identification of enzymes involved in CLA metabolism

• Published in: Brazilian journal of microbiology Vol. 52; no. 3; pp. 1557 - 1563
• Main Authors: Kuhl, Gabriela Christina, Mazzon, Ricardo Ruiz, Duarte, Rubens Tadeu Delgado, De Dea Lindner, Juliano
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01-09-2021; Springer Nature B.V
• Subjects: Biomedical and Life Sciences; Cheese - microbiology; Dairy products; Enzymes; Fermented food; Fermented milk products; Food chains; Food Microbiology; Food Microbiology - Short Communication; Genome, Bacterial; Genomes; Italy; Lactobacilli; Lactobacillus delbrueckii; Lactobacillus delbrueckii - genetics; Life Sciences; Linoleic acid; Linoleic Acids, Conjugated - metabolism; Medical Microbiology; Metabolism; Microbial Ecology; Microbial Genetics and Genomics; Microbiology; Milk; Mycology; Nucleotide sequence; Oleate hydratase; Italy; ssp; Lactic acid bacteria; Genome sequencing; Bioprocess; 11 CLA; 9; Functional food; cis-9, trans-11 CLA; Lactobacillus ssp
• ISSN: 1517-8382; 1678-4405
• DOI: 10.1007/s42770-021-00479-2

Several
Lactobacillus
ssp. are recognized as potential conjugated linoleic acid (CLA) producers. We have previously reported the ability of a range of
Lactobacillus delbrueckii
subsp.
bulgaricus
strains to produce CLA in fermented milk, being a potential candidate for the fermented dairy food chain. This study reports the draft genome sequence of
L. bulgaricus
strain LBP UFSC 2230, isolated from Italian Grana Padano cheese. Draft genome sequence originated in a total of 4,310,842 paired-end reads that were quality trimmed and assembled into 135 contigs with a total length of 604,745,873 bp, including 2086 protein coding genes and an average GC content of 49.7%. Draft genome sequence represents an important tool to identify the enzymes involved in this strain’s CLA metabolism. We identified a gene encoding an enzyme involved in biohydrogenation of linoleic acid pathway, oleate hydratase.