Structural Basis for the Antibiotic Activity of Ketolides and Azalides

• Published in: Structure (London) Vol. 11; no. 3; pp. 329 - 338
• Main Authors: Schlünzen, Frank, Harms, Jörg M, Franceschi, Francois, Hansen, Harly A.S, Bartels, Heike, Zarivach, Raz, Yonath, Ada
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01-03-2003
• Subjects: Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - metabolism; antibiotics; azalides; azithromycin; Azithromycin - chemistry; Azithromycin - metabolism; Erythromycin - analogs & derivatives; Erythromycin - chemistry; Erythromycin - metabolism; Ketolides; peptidyl transferase center; ribosome; Ribosomes - metabolism; RNA, Ribosomal; Structure-Activity Relationship; ketolides; ribosome; azithromycin; antibiotics; azalides; peptidyl transferase center
• ISSN: 0969-2126; 1878-4186
• DOI: 10.1016/S0969-2126(03)00022-4

The azalide azithromycin and the ketolide ABT-773, which were derived by chemical modifications of erythromycin, exhibit elevated activity against a number of penicillin- and macrolide-resistant pathogenic bacteria. Analysis of the crystal structures of the large ribosomal subunit from
Deinococcus radiodurans complexed with azithromycin or ABT-773 indicates that, despite differences in the number and nature of their contacts with the ribosome, both compounds exert their antimicrobial activity by blocking the protein exit tunnel. In contrast to all macrolides studied so far, two molecules of azithromycin bind simultaneously to the tunnel. The additional molecule also interacts with two proteins, L4 and L22, implicated in macrolide resistance. These studies illuminated and rationalized the enhanced activity of the drugs against specific macrolide-resistant bacteria.