Biomarker responses induced by bisphenol A on spermatogenesis in a Neotropical teleost fish are temperature-dependent

• Published in: Ecotoxicology and environmental safety Vol. 224; p. 112670
• Main Authors: Ribeiro, Yves Moreira, Weber, André Alberto, Paschoalini, Alessandro Loureiro, Moreira, Davidson Peruci, Sales, Camila Ferreira, Almeida, Thais Victória Pires de, Neres, Mirra Angelina, Bazzoli, Nilo, Rizzo, Elizete
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Inc 01-11-2021; Elsevier
• Subjects: Apoptosis; Astyanax bimaculatus; Cell proliferation; Endocrine disruption; Reproductive toxicology; Sex steroids; Cell proliferation; Endocrine disruption; Reproductive toxicology; Sex steroids; Astyanax bimaculatus; Apoptosis
• ISSN: 0147-6513; 1090-2414
• DOI: 10.1016/j.ecoenv.2021.112670

Bisphenol A (BPA) is an organic synthetic compound used in the plastic industry with endocrine disrupting activity. Although it is frequently found in surface waters, few studies have investigated its impact on fish gametogenesis, particularly when associated with natural stressors. In this regard, the present study evaluated BPA toxicity on spermatogenesis in the lambari Astyanax bimaculatus under controlled conditions and its interactive effects with water temperature. Adult specimens were exposed in duplicate to 40 μg/L and 400 μg/L BPA at 23 °C and 28 °C for 21 days; the control group did not receive BPA. Testicular samples were collected and analyzed using different cellular and molecular techniques. The results showed a significant reduction in the gonadosomatic index in the BPA-treated groups at both temperatures. A decrease in the testicular levels of 11-ketotestosterone was observed in the 400 μg/L BPA group at 23 °C, 17β-estradiol increased significantly in the treated groups at 28 °C, and vitellogenin showed no difference between the treatments. The morphometric analysis of spermatogenesis revealed a significant increase in the proportion of spermatogonia, spermatocytes, and Sertoli cells in the treated groups, with a higher proportion at 23 °C than at 28 °C. Otherwise, the proportion of spermatozoa was significantly lower in the BPA-treated groups, with a greater reduction at 23 °C. In addition, BPA also stimulated spermatogonial proliferation in the treated groups, but apoptosis was significantly increased in spermatids at 23 °C. Testis-ova, cell degeneration, and chromatin alterations in spermatids and Sertoli cells were observed in the germinal epithelium of the BPA-treated groups. The integrated biomarker response (IBR) index revealed that the analyzed endpoints are suitable for assessing estrogenic contamination. Taken together, our results indicate that the interactive effects of BPA and temperature contribute to the impairment of spermatogenesis in A. bimaculatus with more severe effects observed on sperm production at 23 °C than at 28 °C.
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•Reproductive toxicity of BPA was evaluated in testis of the lambari Astyanax bimaculatus.•BPA downregulates 11-KT at 23 °C and upregulates E2 at 28 °C.•BPA affects germ cells apoptosis in a temperature-dependent manner.•Interactive effects of BPA and temperature impair spermatogenesis.•IBR index revealed induction of BPA responses at 28 °C and inhibition at 23 °C.