First detection of emerging HoBi-like Pestivirus (BVD-3) among some persistently infected dairy cattle herds in Egypt

• Published in: Tropical animal health and production Vol. 54; no. 6; p. 336
• Main Authors: Afify, Ahmed F., Hassanien, Rabab T., Abdelmegeed, Hala K., Abouelyazeed, Ebtsam A., Ali, M. H., Abdelwahed, Dina A., Behour, Tahani S.
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01-12-2022; Springer Nature B.V
• Subjects: 5' Untranslated Regions; Animals; Biomedical and Life Sciences; Bovine Virus Diarrhea-Mucosal Disease - epidemiology; Calves; Cattle; Cattle Diseases - epidemiology; Dairy cattle; Diarrhea; Diarrhea Viruses, Bovine Viral; Disease; Egypt - epidemiology; Enzyme-linked immunosorbent assay; Enzymes; Farms; Genomes; Infections; Life Sciences; Nucleotide sequence; Nucleotides; Pestivirus; Pestivirus - genetics; Pestivirus Infections - epidemiology; Pestivirus Infections - veterinary; Proteins; Regular; Regular Articles; Research centers; Serology; Strains (organisms); Veterinary Medicine/Veterinary Science; Viruses; Zoology; Egypt; HoBi-like Pestivirus; BVD; Persistent infection; RT-PCR; ELISA
• ISSN: 0049-4747; 1573-7438
• DOI: 10.1007/s11250-022-03332-2

Bovine viral diarrhoea virus (BVDV) is a serious veterinary health concern worldwide. We conducted this study to determine the prevalence of persistent infections (PI) and identify the current strain among some dairy cattle herds in Egypt. A total of 240 serum samples were collected from six Egyptian provinces. Between 2019 and 2020, samples were tested by Enzyme linked immunosorbent assay (ELISA) for detection of PI animals, and then molecular characterization was performed. Six calves were found PI with a prevalence of 2.5% (6/240). Using molecular characterization, HoBi-like Pestivirus (BVD-3) was successfully identified in Egypt for the first time. Based on the BVD-3 reference strains on Genbank, the detected strains had an identity ranging from 98.8 to 99.6%. Partial nucleotide sequence of the 5′UTR gene for six tested samples was submitted to Genbank with accessions: OM324396, OM324397, OM324398, OM324399, OM3243100, and OM3243101.