Male zebra finches exposed to lead (Pb) during development have reduced volume of song nuclei, altered sexual traits, and received less attention from females as adults

• Published in: Ecotoxicology and environmental safety Vol. 210; p. 111850
• Main Authors: Goodchild, Christopher G., Beck, Michelle L., VanDiest, Isaac, Czesak, Frankie N., Lane, Samuel J., Sewall, Kendra B.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Inc 01-03-2021; Elsevier
• Subjects: Animals; Attention; Bill coloration; Cognition - drug effects; Dietary lead; Environmental Pollutants - toxicity; Female; Finches - physiology; Lead - toxicity; Learning - drug effects; Male; Mate choice; Neurotoxicity; Phenotype; Pigmentation - drug effects; Song learning; Song nuclei; Vocalization, Animal - drug effects; Bill coloration; Dietary lead; Neurotoxicity; Song learning; Song nuclei; Mate choice
• ISSN: 0147-6513; 1090-2414
• DOI: 10.1016/j.ecoenv.2020.111850

Lead (Pb) is a pervasive global contaminant that interferes with sensitive windows for neurological development and causes oxidative damage to tissues. The effects of moderate and high exposure to Pb have been well-studied in birds, but whether low-level early-life exposure to Pb influences adult phenotype remains unclear. Female songbirds use a male's song and coloration to discriminate between high- and low-quality males. Therefore, if early-life exposure to Pb disrupts song learning ability or shifts the allocation of antioxidant pigments away from colorful secondary sexual traits, male birds exposed to Pb may be less attractive to females. We exposed developing zebra finches (Taeniopygia guttata) to Pb-contaminated drinking water (100 or 1000 parts per billion [ppb]) after hatching (days 0–100). Once male finches reached adulthood (120–150 days post hatch), we measured song learning ability, coloration of bill and cheek patches, and volume of song nuclei in the brain. We also measured female preference for Pb-exposed males relative to control males. Finally, we measured motoric and spatial cognitive performance in male and female finches to assess whether cognitive traits differed in their sensitivity to Pb exposure. Male zebra finches exposed to 1000 ppb Pb had impaired song learning ability, reduced volume of song nuclei, bills with less redness and received less attention from females. Additionally, Pb exposure impaired motoric performance in both male and female finches but did not affect performance in a spatial cognitive task. Adult finches exposed to Pb-contaminated water had higher blood-Pb levels, though in all cases blood-Pb levels were below 7.0 µg dL−1. This study suggests that low-level exposure to Pb contributes to cognitive deficits that persist into adulthood and may indirectly influence fitness by altering secondary sexual traits and reducing male attractiveness.
•Developmental Pb exposure impaired song learning in male zebra finches.•Developmental Pb exposure reduced song nuclei in the brains of male zebra finches.•Developmental Pb exposure reduced bill redness in male zebra finches.•Male zebra finches exposed to Pb received less attention from females.