In-vivo wound healing activity of a novel composite sponge loaded with mucilage and lipoidal matter of Hibiscus species

• Published in: Biomedicine & pharmacotherapy Vol. 135; p. 111225
• Main Authors: Bakr, Riham O., Amer, Reham I., Attia, Dalia, Abdelhafez, Mai M., Al-Mokaddem, Asmaa K., El-Gendy, Abd El-Nasser G., El-Fishawy, Ahlam M., Fayed, Marwa A.A., Gad, Sameh S.
• Format: Journal Article
• Language: English
• Published: France Elsevier Masson SAS 01-03-2021; Elsevier
• Subjects: Administration, Cutaneous; Animals; Cell Line; Chitosan; Chitosan - administration & dosage; Disease Models, Animal; Hibiscus; Hibiscus - chemistry; Humans; Inflammation Mediators - metabolism; Lipids - administration & dosage; Lipids - isolation & purification; Lipoidal matter; Male; Mucilage; Plant Extracts - administration & dosage; Plant Extracts - isolation & purification; Rats; Rats, Wistar; Re-Epithelialization - drug effects; Skin - drug effects; Skin - injuries; Skin - metabolism; Skin - pathology; Surgical Sponges; Transforming Growth Factor beta - metabolism; Tumor Necrosis Factor-alpha - metabolism; Vascular Endothelial Growth Factor A - metabolism; Wound Healing - drug effects; Wound-healing sponge; Wounds, Penetrating - drug therapy; Wounds, Penetrating - metabolism; Wounds, Penetrating - pathology; Wound-healing sponge; Chitosan; Lipoidal matter; Mucilage; Hibiscus
• ISSN: 0753-3322; 1950-6007
• DOI: 10.1016/j.biopha.2021.111225

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•Hibiscus syriacus extracts have high wound healing activity.•Fatty acids, sterols and mucilage resulted in a potential wound healing effect.•The formulated sponge: Chitosan/Mucilage and petroleum ether extract modulated TNF-α.•Re-epithelization and epidermal remodeling were perfect with the formulated sponge.
Many researches have been undergone to hasten the natural wound healing process. In this study, several Hibiscus species (leaves) were extracted with petroleum ether, methanol, and their mucilage was separated. All the tested species extracts were assessed for their viability percentage using the water-soluble tetrazolium. H.syriacus was the plant of choice to be incorporated in a new drug delivery system and evaluated for its wound healing activity. H.syriacus petroleum ether extract (PEE) showed a high percentage of palmitic and oleic acids while its mucilage demonstrated high glucosamine and galacturonic acid. It was selected to be formulated and pharmaceutically evaluated into three different composite sponges using chitosan in various ratios. Fourier-transformed infrared spectroscopy investigated the chemical interaction between the utilized sponges’ ingredients. Morphological characteristics were evaluated using scanning electron microscopy. H.syriacus composite sponge of mucilage: chitosan (1:5) was loaded with three different concentrations of PEE. Medicated formulations were assessed in rat model of excision wound model. The wound healing ability was clearly proved by the clinical acceleration, histopathological examination, and modulation of correlated inflammatory parameters as tumor necrosis factor in addition to vascular endothelial growth factor suggesting a promising valuable candidate that supports the management of excision wounds using single-dose preparation.