The impact of ABCB1 gene polymorphism and its expression on non-small-cell lung cancer development, progression and therapy – preliminary report

• Published in: Scientific reports Vol. 10; no. 1; p. 6188
• Main Authors: Zawadzka, Izabela, Jeleń, Agnieszka, Pietrzak, Jacek, Żebrowska-Nawrocka, Marta, Michalska, Katarzyna, Szmajda-Krygier, Dagmara, Mirowski, Marek, Łochowski, Mariusz, Kozak, Józef, Balcerczak, Ewa
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 10-04-2020; Nature Publishing Group
• Subjects: 631/208; 631/337; 631/67; 692/308; 692/4017; 692/53; Aged; Antineoplastic Combined Chemotherapy Protocols - pharmacology; Antineoplastic Combined Chemotherapy Protocols - therapeutic use; ATP Binding Cassette Transporter, Subfamily B - blood; ATP Binding Cassette Transporter, Subfamily B - genetics; ATP Binding Cassette Transporter, Subfamily B - metabolism; Blood; Carcinogenesis - genetics; Carcinoma, Non-Small-Cell Lung - blood; Carcinoma, Non-Small-Cell Lung - genetics; Carcinoma, Non-Small-Cell Lung - mortality; Carcinoma, Non-Small-Cell Lung - therapy; Case-Control Studies; Chemotherapy, Adjuvant; Disease Progression; Drug Resistance, Multiple - genetics; Drug Resistance, Neoplasm - genetics; Drug therapy; Female; Follow-Up Studies; Gene polymorphism; Genotyping; Healthy Volunteers; Humanities and Social Sciences; Humans; Kaplan-Meier Estimate; Lung - pathology; Lung - surgery; Lung cancer; Lung Neoplasms - blood; Lung Neoplasms - genetics; Lung Neoplasms - mortality; Lung Neoplasms - therapy; Male; Middle Aged; mRNA; multidisciplinary; Multidrug resistance; Neoplasm Staging; Non-small cell lung carcinoma; Poland - epidemiology; Polymerase chain reaction; Polymorphism, Single Nucleotide; Restriction fragment length polymorphism; Science; Science (multidisciplinary); Single-nucleotide polymorphism; Poland
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-020-63265-4

The
ABCB1
gene belongs to ATP binding cassette (ABC) transporter genes that has been previously implicated in cancer progression and drug response. This study aimed to evaluate the association between the SNP 3435 and the expression of the
ABCB1
gene in lung cancer patients in the Polish population in comparison to clinicopathological parameters and treatment. 150 RNA and 47 DNA samples were isolated from 49 lung cancer cases including both tissue samples and blood taken from the same patients at three time points: diagnosis, 100 days and one year after the surgical intervention. Qualitative and real-time PCR analysis of expression were done, also genotyping by PCR-RFLP. Mutant homozygous TT and allele T are present statistically significantly more frequently in the group of patients with lung cancer. There is no difference with expression level in lung cancer tissue and blood sample taken from the same patients before surgical treatment. On the basis of blood samples analysis it was observed that the expression level of
ABCB1
mRNA was growing in time. Higher levels were marked after 100 days and one year after the surgical intervention. The complementary pharmacological treatment induced higher expression levels of
ABCB1
. The presented data suggest an important role of
ABCB1
in lung cancer, the increasing level of
ABCB1
mRNA which can be connected with induction of multidrug resistance mechanism is also significant, that observation must be confirmed in further analysis.