Circulating tumour cells are associated with histopathological growth patterns of colorectal cancer liver metastases

• Published in: Clinical & experimental metastasis Vol. 40; no. 1; pp. 69 - 77
• Main Authors: Meyer, Y. M., Wilting, S. M., Kraan, J., Olthof, P., Vermeulen, P., Martens, J., Grünhagen, D. J., Sleijfer, S., Verhoef, C.
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01-02-2023; Springer Nature B.V
• Subjects: Biomedical and Life Sciences; Biomedicine; Cancer Research; Chemotherapy; Colorectal cancer; Colorectal carcinoma; Colorectal Neoplasms - pathology; Growth patterns; Hematology; Humans; Liver; Liver cancer; Liver Neoplasms - secondary; Metastases; Metastasis; Neoplastic Cells, Circulating - pathology; Oncology; Prediction models; Prognosis; Regression analysis; Research Paper; Retrospective Studies; Statistical analysis; Surgical Oncology; Tumors; Histopathological growth patterns; Colorectal liver metastases; Circulating tumour cells; Prediction
• ISSN: 0262-0898; 1573-7276
• DOI: 10.1007/s10585-022-10191-6

Histopathological Growth Patterns (HGPs) have prognostic and predictive value in patients with Colorectal Liver Metastases (CRLM). This study examined whether preoperative measurement of Circulating Tumour Cells (CTCs) is associated with HGP. CTCs were prospectively enumerated in 7.5 ml of blood using the FDA-approved CellSearch system in patients who underwent local treatment of CRLM with curative intent between 2008 and 2021. All CTC samples were collected on the day of local treatment. Patients treated with neoadjuvant chemotherapy for CRLM or with extrahepatic disease at the time of CTC sampling were excluded. HGP was scored retrospectively following the current consensus guidelines. The association between CTCs and HGP was investigated through multivariable logistic regression. Data were available for 177 patients, desmoplastic HGP (dHGP) was observed in 34 patients (19%). There were no statistically significant differences in patient and tumour characteristics between dHGP and non-dHGP at baseline. Patients with dHGP had longer overall – and disease-free survival (logrank p = 0.003 and 0.003, respectively) compared to patients with non-dHGP. CTCs were not detected in 25(74%) of dHGP patients and in 68(48%) of non-dHGP patients (chi-squared p = 0.006). Preoperative absence of CTCs was the only significant predictor for dHGP in multivariable logistic regression (Odds Ratio 2.7, 95%CI 1.1–6.8, p = 0.028), Table
3
. Preoperative absence of CTCs is associated with dHGP in chemo naive CRLM patients without extrahepatic disease. Based on our results, CTC count alone is not sufficient to preoperatively identify HGPs, but integration of CTC count in multivariable prediction models may aid the preoperative identification of HGPs of CRLM.