Protective effect of Polygonatum sibiricum Polysaccharide on D-galactose-induced aging rats model

• Published in: Scientific reports Vol. 10; no. 1; p. 2246
• Main Author: Zheng, Shaoyan
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 10-02-2020; Nature Publishing Group
• Subjects: 631/181/2475; 692/4017; 96/1; 96/21; 96/63; Aging; Aging - drug effects; Animal tissues; Animals; Calcium - metabolism; D-Galactose; Dietary Carbohydrates - adverse effects; Femur; Fibroblast growth factor 23; Fibroblast Growth Factors - metabolism; FOXO3 protein; Galactose; Galactose - adverse effects; Gene expression; Glucuronidase - metabolism; Humanities and Social Sciences; Immunoregulation; Kidneys; Klotho protein; Male; Meninges; mRNA; multidisciplinary; Oxidative stress; Oxidative Stress - drug effects; Phosphorus; Phosphorus - metabolism; Phytochemicals - pharmacology; Polygonatum; Polygonatum - chemistry; Polysaccharides; Polysaccharides - pharmacology; Protective Agents - pharmacology; Proteins; Rats; Rats, Sprague-Dawley; Renal cortex; Science; Science (multidisciplinary)
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-020-59055-7

The polysaccharide of Polygonatum sibiricum (PSP)is one of the main active ingredients of Polygonatum Polygonatum in Liliaceae. It has anti-tumor, anti-aging, immune regulation, and anti-oxidative effects. Recent studies have shown that the Klotho gene and fibroblast growth factor-23 (FGF-23) have a common receptor, which is closely related to aging and highly expressed in kidney and meninges. Our study aimed to investigate the anti-aging effect of PSP on D-galactose-induced rats and its mechanism. D-galactose (120 mg Kg
-1
) and PSP (100 mg Kg
-1
) was used to intervene in rats, respectively. Then The changes of indexes of the natural aging-like model rats before and after PSP intervention were observed. We found that PSP could significantly improve the learning and memory abilities of rats and reverse the pathological changes of kidney tissues in rats. At the same time, PSP up-regulated the expression of Klotho mRNA and Klotho protein in the renal cortex, down-regulated the expression of FOXO3a mRNA and p-FOXO3a protein in renal tissue, and inhibited the expression of FGF-23 protein in the femur. Our studies suggest that PSP may play a role by regulating the Klotho-FGF23 endocrine axis, alleviating oxidative stress, and balancing calcium and phosphorus metabolism.