The copy number variation of DMBT1 gene effects body traits in two Chinese cattle breeds

• Published in: 3 Biotech Vol. 12; no. 4; p. 93
• Main Authors: Zheng, Li, Xu, Jiawei, Liu, Xian, Zhang, Zijing, Zhong, Jialin, Wen, Yifan, Yao, Zhi, Yang, Peng, Wang, Eryao, Chen, Fuying, Huang, Weihong, Qi, Zengfang, Yang, Guojie, Lei, Chuzhao, Chen, Hong, Ru, Baorui, Huang, Yongzhen
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01-04-2022; Springer Nature B.V
• Subjects: Agriculture; Bioinformatics; Biomarkers; Biomaterials; Biotechnology; Body height; Body length; Body size; Brain tumors; Calves; Cancer Research; Cattle; Chemistry; Chemistry and Materials Science; Chest; Copy number; Differentiation; DMBT1 protein; Frequency analysis; Frequency distribution; Gene expression; Genomes; Marker-assisted selection; Mutation; Myoblasts; Original; Original Article; Scavenger receptors; Stem Cells; Tuberculosis; Association analysis; CNV; Cattle; Body size; MAS
• ISSN: 2190-572X; 2190-5738
• DOI: 10.1007/s13205-022-03124-3

Copy number variations (CNVs) belong to mutations in the genome level with loci in the region of genic or intergenic. It is through different effects (such as position effect and dose effect) that influence complex traits and diseases. Deleted in Malignant Brain Tumors 1 (
DMBT1
) gene is a member of the scavenger receptor cysteine-rich super family. In cattle, this gene has been associated with the susceptibility to bovine tuberculosis. In this study, a new CNV was found in
DMBT1
gene of Chinese cattle breeds and tested in two different Chinese cattle breeds (Jiaxian red and Pinan) for frequency distribution analysis. Besides, the body size data such as body length, body height, chest girth, chest width, rump length, and rump girth for Jiaxian (JX) and Pinan (PN) cattle were collected and associated with the newly identified CNV. The CNV was significantly associated with the body length and chest girth of JX cattle, and the rump length of PN cattle (
P
 < 0.05). Furthermore, the expression profile of the
DMBT1
gene was tested in calves’ tissues and the myoblasts differentiation. It was found that the
DMBT1
gene expression was high in tuberculosis susceptible tissues (liver and lungs) at the calf stage and high in myoblast early differentiation. These tests were done using the qPCR method. As the result, the CNV of
DMBT1
gene could be used as a candidate marker for bovine growth and health in marker-assisted selection (MAS) breeding.