Plant Cytokinesis Is Orchestrated by the Sequential Action of the TRAPPII and Exocyst Tethering Complexes

• Published in: Developmental cell Vol. 29; no. 5; pp. 607 - 620
• Main Authors: Rybak, Katarzyna, Steiner, Alexander, Synek, Lukas, Klaeger, Susan, Kulich, Ivan, Facher, Eva, Wanner, Gerhard, Kuster, Bernhard, Zarsky, Viktor, Persson, Staffan, Assaad, Farhah F.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 09-06-2014
• Subjects: Arabidopsis - cytology; Arabidopsis - physiology; Arabidopsis Proteins - metabolism; Cytokinesis - physiology; Cytoplasmic Vesicles - metabolism; Exocytosis - physiology; Growth Plate - cytology; Growth Plate - metabolism; Microtubules - metabolism; Models, Molecular; Vesicular Transport Proteins - metabolism
• ISSN: 1534-5807; 1878-1551
• DOI: 10.1016/j.devcel.2014.04.029

Plant cytokinesis is initiated in a transient membrane compartment, the cell plate, and completed by a process of maturation during which the cell plate becomes a cross wall. How the transition from juvenile to adult stages occurs is poorly understood. In this study, we monitor the Arabidopsis transport protein particle II (TRAPPII) and exocyst tethering complexes throughout cytokinesis. We show that their appearance is predominantly sequential, with brief overlap at the onset and end of cytokinesis. The TRAPPII complex is required for cell plate biogenesis, and the exocyst is required for cell plate maturation. The TRAPPII complex sorts plasma membrane proteins, including exocyst subunits, at the cell plate throughout cytokinesis. We show that the two tethering complexes physically interact and propose that their coordinated action may orchestrate not only plant but also animal cytokinesis.
•TRAPPII and exocyst complexes appear sequentially at the cell plate and cross wall•The TRAPPII complex is required for cell plate biogenesis and protein sorting•The exocyst is required for cell plate maturation•TRAPPII and exocyst interact physically
Plant cytokinesis initiates at a transient membrane compartment—the cell plate—that matures to become a cross wall. Rybak et al. show that TRAPPII and exocyst complexes act sequentially during cytokinesis. TRAPPII is required for cell plate biogenesis and protein sorting, whereas the exocyst is needed for cell plate maturation.