A bioactive fraction of Pterocarpus santalinus inhibits adipogenesis and inflammation in 3T3-L1 cells via modulation of PPAR-γ/SREBP-1c and TNF-α/IL-6

• Published in: 3 Biotech Vol. 11; no. 5; p. 233
• Main Authors: Reddy Sankaran, Karunakaran, Ganjayi, Muni Swamy, Oruganti, Lokanatha, Chippada, Appa Rao, Meriga, Balaji
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01-05-2021; Springer Nature B.V
• Subjects: Adipogenesis; Adiponectin; Agriculture; Biocompatibility; Bioinformatics; Biological activity; Biomaterials; Biotechnology; Cancer Research; Cell culture; Chemistry; Chemistry and Materials Science; Chloroform; Down-regulation; Gene expression; Glycerol; Inflammation; Interleukin 6; Leptin; Lipids; Lipopolysaccharides; Obesity; Original; Original Article; Peroxisome proliferator-activated receptors; Proteins; Pterocarpus santalinus; Stem Cells; Sterol regulatory element-binding protein; Tumor necrosis factor-TNF; Tumor necrosis factor-α; Oil-red O stain; Inflammation; Proinflammatory cytokines; Adipokines; Lipolysis; Adipogenesis
• ISSN: 2190-572X; 2190-5738
• DOI: 10.1007/s13205-021-02771-2

Pterocarpus santalinus
has huge demand owing to its commercial and medicinal value. However, there are limited research studies on its therapeutic activity against obesity and obesity-induced inflammation and underlying mechanism of action. Therefore, in the present study, chloroform bioactive fraction of
P. santalinus
(CFP) was isolated and evaluated for its activity against adipogenesis and adipogenesis-induced inflammation in 3T3-L1 cell culture model. LC–MS/MS analysis of CFP was performed to identify the compounds present. CFP-treated 3T3-L1 cells (50, 100 and 200 μg/ml) have significantly (
p
 < 0.01 or < 0.05) enhanced glycerol release and adiponectin level, but reduced lipid accumulation and leptin, and MTT assay demonstrated CFP was non-toxic till a dose of 300 µg/ml at 24 and 48 h. A considerable reduction in tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) levels was witnessed in lipopolysaccharide (LPS)-induced 3T3-L1 cells with CFP treatment in dose-dependent manner. Gene expression studies demonstrated down-regulation of mRNA expression of peroxisome proliferator-activated receptor-γ (PPAR-γ), sterol regulatory element-binding protein-1c (SREBP-1c), leptin, TNF-α and IL-6 but up-regulation of adiponectin and uncoupling protein-1 (UCP-1) and the same trend was observed in protein expression also. In conclusion, it is suggested that CFP could be beneficial to treat obesity and associated inflammation.