Letter regarding “Validation of a cage‐side agglutination card for Dal blood typing in dogs” – Validation of agglutination card kit or simple comparison to gel column method for canine Dal blood typing?

• Published in: Journal of veterinary internal medicine Vol. 37; no. 4; pp. 1296 - 1297
• Main Authors: Giger, Urs, Freeman, Kathleen P.
• Format: Journal Article
• Language: English
• Published: Hoboken, USA John Wiley & Sons, Inc 01-07-2023; Wiley
• Subjects: Antibodies; Antigens; Blood & organ donations; Blood groups; Dogs; Laboratories; Letter to the Editor; Letters to the Editor; Veterinary medicine; Canada; Montreal Quebec Canada
• ISSN: 0891-6640; 1939-1676
• DOI: 10.1111/jvim.16788

Pending manufacturer instructions, the sample requirements (e.g., acceptable anticoagulant, hematocrit range, storage time of samples), ease and adequacy of standard operating procedure, accuracy of testing based upon method, repeatability of results over time, required operator training, variation in interpretation of test results by untrained and trained operators, and several statistical analyses should be included. Additional studies carried out at a different department at Montreal University characterized a monoclonal anti-Dal antibody in 2020. Because monoclonal antibodies are more easily standardized, its use would have been ideal for comparison with the polyclonal alloantibodies and would likely better reflect a gold standard method. 7 For optimal performance of the gel column test to be considered a gold standard, the antibody (sera) should be in and not only on top of the gel. [...]a clinical comparison of 2 methods should not be confused with a formal validation study.