A role for topoisomerase III in a recombination pathway alternative to RuvABC

• Published in: Molecular microbiology Vol. 58; no. 1; pp. 80 - 101
• Main Authors: Lopez, Christopher R., Yang, Shirley, Deibler, Richard W., Ray, Starlight A., Pennington, Jeanine M., DiGate, Russell J., Hastings, P. J., Rosenberg, Susan M., Zechiedrich, E. Lynn
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Science Ltd 01-10-2005; Blackwell Science; Blackwell Publishing Ltd
• Subjects: Adenosine Triphosphatases - genetics; Adenosine Triphosphatases - physiology; Bacteria; Bacterial Proteins - genetics; Bacterial Proteins - physiology; Biological and medical sciences; Chemical compounds; Deoxyribonucleic acid; DNA; DNA Helicases - genetics; DNA Helicases - physiology; DNA Topoisomerases, Type I - genetics; DNA Topoisomerases, Type I - physiology; DNA, Bacterial - metabolism; DNA, Catenated - analysis; DNA, Superhelical - metabolism; Endodeoxyribonucleases - genetics; Endodeoxyribonucleases - physiology; Escherichia coli - enzymology; Escherichia coli - genetics; Escherichia coli Proteins - genetics; Escherichia coli Proteins - physiology; Fundamental and applied biological sciences. Psychology; Gene Deletion; Genomics; Microbiology; Mutation; Rec A Recombinases - genetics; Rec A Recombinases - physiology; Recombination, Genetic; RecQ Helicases; Serine Endopeptidases - genetics; Temperature; Transduction, Genetic; Microbiology; Recombination
• ISSN: 0950-382X; 1365-2958
• DOI: 10.1111/j.1365-2958.2005.04812.x

Summary
The physiological role of topoisomerase III is unclear for any organism. We show here that the removal of topoisomerase III in temperature sensitive topoisomerase IV mutants in Escherichia coli results in inviability at the permissive temperature. The removal of topoisomerase III has no effect on the accumulation of catenated intermediates of DNA replication, even when topoisomerase IV activity is removed. Either recQ or recA null mutations, but not helD null or lexA3, partially rescued the synthetic lethality of the double topoisomerase III/IV mutant, indicating a role for topoisomerase III in recombination. We find a bias against deleting the gene encoding topoisomerase III in ruvC53 or ΔruvABC backgrounds compared with the isogenic wild‐type strains. The topoisomerase III RuvC double mutants that can be constructed are five‐ to 10‐fold more sensitive to UV irradiation and mitomycin C treatment and are twofold less efficient in transduction efficiency than ruvC53 mutants. The overexpression of ruvABC allows the construction of the topoisomerase III/IV double mutant. These data are consistent with a role for topoisomerase III in disentangling recombination intermediates as an alternative to RuvABC to maintain the stability of the genome.