Bioavailability of vitamin E compounds and the effect of supplementation on release of superoxide and hydrogen peroxide by bovine neutrophils

• Published in: Journal of dairy science Vol. 80; no. 1; p. 187
• Main Authors: Hidiroglou, M, Batra, T R, Zhao, X
• Format: Journal Article
• Language: English
• Published: United States 01-01-1997
• Subjects: alpha-Tocopherol - analogs & derivatives; Animals; Biological Availability; Cattle; Erythrocytes - metabolism; Female; Hydrogen Peroxide - blood; Neutrophils - drug effects; Neutrophils - metabolism; Pregnancy; Superoxides - blood; Tetradecanoylphorbol Acetate - pharmacology; Tocopherols; Vitamin E - administration & dosage; Vitamin E - analogs & derivatives; Vitamin E - blood; Vitamin E - pharmacokinetics
• ISSN: 0022-0302
• DOI: 10.3168/jds.S0022-0302(97)75926-5

The effects of daily supplementation of 1000 IU of d-alpha- or dl-alpha-tocopherol acetate to Holstein cows from drying off to 8 wk after calving were studied 1) to compare the bioavailability of d-alpha- and dl-alpha-tocopherol acetate by monitoring vitamin E concentrations in plasma, red blood cells, and neutrophils and 2) to determine the effect of d-alpha- and dl-alpha-tocopherol acetate supplementation on O2- and H2O2 release by neutrophils. Supplementation with d-alpha- or dl-alpha-tocopherol acetate increased vitamin E concentrations in blood plasma, red blood cells, and neutrophils; the d-alpha form was more effective than was the dl-alpha form. Supplementation with d-alpha- or dl-alpha-tocopherol acetate had no effect on the release of O2- and H2O2 by neutrophils, suggesting no harmful effects on surrounding tissues from oxygen-free radicals produced by neutrophils.