Influence of Xenon on Survival of Sperm of Common Frog Rana temporaria during Slow Freezing

• Published in: Bulletin of experimental biology and medicine Vol. 171; no. 5; pp. 596 - 600
• Main Authors: Kaurova, S. A., Shvirst, N. E., Shishova, N. V., Uteshev, V. K., Fesenko, E. E.
• Format: Journal Article
• Language: English
• Published: New York Springer US 01-09-2021; Springer; Springer Nature B.V
• Subjects: Analysis; Animals; Biological products; Biomaterials; Biomedical and Life Sciences; Biomedicine; Biophysics and Biochemistry; Cell Biology; Cell Survival - drug effects; Cryopreservation - methods; Cryoprotective Agents - pharmacology; Dimethylformamide; Freezing; Freezing - adverse effects; Frogs; Internal Medicine; Laboratory Medicine; Male; Pathology; Rana temporaria; Semen Analysis - veterinary; Semen Preservation - adverse effects; Semen Preservation - methods; Semen Preservation - veterinary; Sperm; Sperm Motility - drug effects; Spermatozoa; Spermatozoa - drug effects; Spermatozoa - physiology; Sucrose; Survival; Xenon; Xenon - pharmacology; xenon; slow freezing; pressure; amphibian spermatozoa; cryopreservation
• ISSN: 0007-4888; 1573-8221
• DOI: 10.1007/s10517-021-05276-3

We studied the effect of xenon on the survival rate of the spermatozoa of the common frog
Rana temporaria
during slow freezing with saturation of the suspension with xenon at a pressure of up to 1.2 bar. The cryoprotective properties of xenon were analyzed in comparison with nitrogen. No specific cryoprotective effect of xenon was revealed. Viability of spermatozoa pretreated with xenon at atmospheric pressure (0 bar) or under excess pressure of 0.6 bar and frozen in a cryoprotective medium with dimethylformamide, sucrose, and BSA did not differ significantly. The use of overpressure of xenon of 1.0 or 1.2 bar in the pretreatment and freezing process significantly impaired viability of the biomaterial.