Distinct in vitro interaction pattern of dopamine receptor subtypes with adaptor proteins involved in post-endocytotic receptor targeting

• Published in: FEBS letters Vol. 556; no. 1; pp. 276 - 280
• Main Authors: Heydorn, Arne, Søndergaard, Birgitte P, Hadrup, Niels, Holst, Birgitte, Haft, Carol Renfrew, Schwartz, Thue W
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 02-01-2004
• Subjects: Animals; Carrier Proteins - genetics; Carrier Proteins - metabolism; Dopamine receptor; Endocytosis - physiology; Escherichia coli - genetics; Ezrin–radixin–moesin-binding phosphoprotein 50; Glutathione Transferase - metabolism; Humans; N-Ethylmaleimide-sensitive factor; N-Ethylmaleimide-Sensitive Proteins; Phosphoproteins - genetics; Phosphoproteins - metabolism; Protein Isoforms; Protein sorting; Rats; Receptors, Dopamine - chemistry; Receptors, Dopamine - genetics; Receptors, Dopamine - metabolism; Recombinant Fusion Proteins - chemistry; Recombinant Fusion Proteins - genetics; Recombinant Fusion Proteins - metabolism; Signal Transduction; Sodium-Hydrogen Exchangers; Sorting nexin 1; Sulfur Radioisotopes; Vesicular Transport Proteins; Sorting nexin 1; N-Ethylmaleimide-sensitive factor; Protein sorting; Ezrin–radixin–moesin-binding phosphoprotein 50; Dopamine receptor
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/S0014-5793(03)01431-5

The mechanisms underlying targeted sorting of endocytosed receptors for recycling to the plasma membrane or degradation in lysosomes are poorly understood. In this report, the C-terminal tails of the five dopamine receptors (D1–D5) were expressed as glutathione
S-transferase (GST) fusion proteins and studied for their interaction with ezrin–radixin–moesin-binding phosphoprotein 50 (EBP50) and
N-ethylmaleimide-sensitive factor (NSF), which are known to be involved in post-endocytic recycling of receptors back to the plasma membrane, and with sorting nexin 1 (SNX1), known to be involved in targeting receptors to lysosomal degradation. EBP50 did not bind any of the dopamine receptor tails. NSF bound strongly to D1 and D5 and only weakly to D2, D3 and D4. However, SNX1 clearly distinguished between D1 and D5, as only D5 bound strongly to this protein. This report shows that there are distinct interaction patterns for NSF and SNX1 to the various dopamine receptor subtypes.