Methanolic Phoenix dactylifera L. Extract Ameliorates Cisplatin-Induced Hepatic Injury in Male Rats

• Published in: Nutrients Vol. 14; no. 5; p. 1025
• Main Authors: Gad El-Hak, Heba Nageh, Mahmoud, Hany Salah, Ahmed, Eman A, Elnegris, Heba M, Aldayel, Tahany Saleh, Abdelrazek, Heba M A, Soliman, Mohamed T A, El-Menyawy, Menna Allah I
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 28-02-2022; MDPI
• Subjects: Actin; Animals; Antioxidants; Apoptosis; Biomarkers; Cancer therapies; Chemical and Drug Induced Liver Injury - drug therapy; Chemical and Drug Induced Liver Injury - prevention & control; Chemotherapy; Cisplatin; Cisplatin - toxicity; Cyclooxygenase-2; Cytochrome; Cytokines; date flesh; Herbal medicine; Immunohistochemistry; Inflammation; inflammatory markers; Kinases; Laboratory animals; Liver; Male; Males; Metabolism; Metabolites; Methanol; Muscles; NF-κB protein; Oxidants; Oxidative stress; Oxidizing agents; Phoeniceae; Plant Extracts - pharmacology; Proteins; Rats; Smooth muscle; Tumor necrosis factor-TNF; United States > US; Egypt; inflammatory markers; cisplatin; antioxidants; date flesh; liver; oxidative stress
• ISSN: 2072-6643; 2072-6643
• DOI: 10.3390/nu14051025

This study investigated the ameliorative potential of methanolic date flesh extract (MDFE) against cisplatin-induced hepatic injury. Twenty male rats (weighing 180-200 g) were allocated into four groups: control; date flesh (DF) group (oral 600 mg/kg MDFE for 21 days); Cis group (7.5 mg/kg i.p. at day 16); and date flesh/cisplatin (DF/Cis) group (oral 600 mg/kg MDFE for 21 days and 7.5 mg/kg i.p. at day 16). Hepatic biochemical parameters in sera, and inflammatory and oxidant/antioxidant hepatic biomarkers were estimated. Hepatic histological changes and the immunohistochemistry of cyclooxygenase-2 (COX-2), nuclear factor kappa B (NF-κB), and alpha smooth muscle actin (α-SMA) were assessed. Pretreatment with MDFE decreased Cis-triggered liver biochemical parameters, oxidative stress, inflammatory biomarkers, and histological damage. Moreover, MDFE treatment reduced Cis-induced hepatic NF-κB, COX-2, and α-SMA protein expression. MDFE exerted a hepatoprotective effect when used concomitantly with Cis. Its effect was mediated via its antioxidant and anti-inflammatory ingredients.