Promising Potential of Crude Polysaccharides from Sparassis crispa against Colon Cancer: An In Vitro Study

• Published in: Nutrients Vol. 13; no. 1; p. 161
• Main Authors: Nowacka-Jechalke, Natalia, Nowak, Renata, Lemieszek, Marta Kinga, Rzeski, Wojciech, Gawlik-Dziki, Urszula, Szpakowska, Nikola, Kaczyński, Zbigniew
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 06-01-2021; MDPI
• Subjects: anti-inflammatory; Anti-Inflammatory Agents - pharmacology; anticancer activity; Antineoplastic Agents - pharmacology; antioxidant; Antioxidants; Antioxidants - pharmacology; Biological activity; Caco-2 Cells; Carbohydrates; Catalase; Cell proliferation; Chemical composition; Chemical compounds; Chemicals; Colon; Colon cancer; Colonic Neoplasms - prevention & control; Colorectal cancer; cyclooxygenase; Cyclooxygenase-2; Ethanol; Food products; Glucans - metabolism; Glucose; Health risks; HT29 Cells; Humans; Inflammation; Invasiveness; Lipoxygenase; Mushrooms; Pharmacology; Phenols; Polyporales - metabolism; Polysaccharides; Polysaccharides - pharmacology; Prostaglandin-Endoperoxide Synthases; Reagents; Saccharides; Sodium; Spectrum analysis; Structural analysis; Tumor cell lines; Xanthine oxidase; β-glucan; Poland; St Louis Missouri; United States > US; Germany; β-glucan; lipoxygenase; antioxidant; anti-inflammatory; cyclooxygenase; anticancer activity; cauliflower mushroom
• ISSN: 2072-6643; 2072-6643
• DOI: 10.3390/nu13010161

The aim of the present study was to evaluate in vitro the beneficial potential of crude polysaccharides from
(CPS) in one of the most common cancer types-colon cancer. The determination of the chemical composition of CPS has revealed that it contains mostly carbohydrates, while proteins or phenolics are present only in trace amounts.
H NMR and GC-MS methods were used for the structural analysis of CPS. Biological activity including anticancer, anti-inflammatory and antioxidant properties of CPS was investigated. CPS was found to be non-toxic to normal human colon epithelial CCD841 CoN cells. Simultaneously, they destroyed membrane integrity as well as inhibited the proliferation of human colon cancer cell lines: Caco-2, LS180 and HT-29. Antioxidant activity was determined by various methods and revealed the moderate potential of CPS. The enzymatic assays revealed no influence of CPS on xanthine oxidase and the inhibition of catalase activity. Moreover, pro-inflammatory enzymes such as cyclooxygenase-2 or lipooxygenase were inhibited by CPS. Therefore, it may be suggested that
is a valuable part of the regular human diet, which may contribute to a reduction in the risk of colon cancer, and possess promising activities encouraging further studies regarding its potential use as chemopreventive and therapeutic agent in more invasive stages of this type of cancer.