Dynamics of antagonistic potency of Rhodobacter capsulatus PG lipopolysaccharide against endotoxin-induced effects

• Published in: Biochemistry (Moscow) Vol. 81; no. 3; pp. 275 - 283
• Main Authors: Kabanov, D. S., Serov, D. A., Zubova, S. V., Grachev, S. V., Prokhorenko, I. R.
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 01-03-2016; Springer; Springer Nature B.V
• Subjects: Biochemistry; Biomedical and Life Sciences; Biomedicine; Bioorganic Chemistry; Cytokines; Cytokines - analysis; Cytokines - metabolism; E coli; Endotoxins; Endotoxins - toxicity; Enzyme-Linked Immunosorbent Assay; Escherichia coli; Escherichia coli - metabolism; Health aspects; Humans; Leukocytes; Leukocytes - cytology; Leukocytes - drug effects; Leukocytes - metabolism; Life Sciences; Lipopolysaccharides; Lipopolysaccharides - pharmacology; Microbiology; Proteobacteria; Rhodobacter capsulatus; Rhodobacter capsulatus - metabolism; Salmonella enterica; cytokine; IFN-γ; IL-1Ra; TNF-α; lipopolysaccharide (endotoxin); IL-1β; IL-8; antagonist; IL-6; IL-10
• ISSN: 0006-2979; 1608-3040
• DOI: 10.1134/S000629791603010X

The dynamics of antagonistic potency of lipopolysaccharide (LPS) isolated from
Rhodobacter capsulatus
PG on the synthesis of proinflammatory (TNF-α, IL-1β, IL-8, IL-6, IFN-γ) and antiinflammatory (IL-10, IL-1Ra) cytokines induced by highly stimulatory endotoxins from
Escherichia coli
or
Salmonella enterica
have been studied. Using human whole blood, we have shown that
R. capsulatus
PG LPS inhibited most pronouncedly the endotoxin-induced synthesis of TNF-α, IL-1β, IL-8, and IL-6 during the first 6 h after endotoxin challenge. Similarly, the endotoxin-induced release of IFN-γ was abolished by
R. capsulatus
PG LPS as well (24 h). In contrast to the above-mentioned cytokines, the relatively weak antagonistic activity of
R. capsulatus
PG LPS against endotoxin-triggered production of IL-6 and IL-8 was revealed. Since
R. capsulatus
PG LPS displays more potent antagonistic activity against deleterious effects of
S. enterica
LPS than those of
E. coli
LPS in the cases of such cytokines as IL-1β (6 and 24 h), IL-6 and IL-8 (4 h), we conclude that the effectiveness of protective action of antagonist is mostly determined by the primary lipid A structure of the employed agonist.