Antioxidative Effects of Olive Polyphenols Compared to Vitamin E in Piglets Fed a Diet Rich in N-3 PUFA

• Published in: Animals (Basel) Vol. 9; no. 4; p. 161
• Main Authors: Leskovec, Jakob, Rezar, Vida, Svete, Alenka Nemec, Salobir, Janez, Levart, Alenka
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 12-04-2019; MDPI
• Subjects: Animals; Antioxidants; Chemical compounds; Deoxyguanosine; Deoxyribonucleic acid; Diet; DNA; Enzymes; Feeds; Hemoglobin; Hogs; hydroxytyrosol; Isoprostanes; Linseed oil; Lipids; Low fat diet; Malondialdehyde; Nutrient deficiency; Oils & fats; olive polyphenols; Oxidative stress; piglets; Plant extracts; Polyphenols; Software; Tocopherol; Urine; Vitamin E; γ-Glutamyltransferase; United Kingdom > UK; United States > US; Germany; piglets; hydroxytyrosol; olive polyphenols; linseed oil; oxidative stress; vitamin E
• ISSN: 2076-2615; 2076-2615
• DOI: 10.3390/ani9040161

The aim of the current study was to assess the antioxidative effects of the dietary supplementation of olive leaf extract (OLE) in different concentrations compared to those of vitamin E in piglets under conditions of dietary n-3 PUFA-induced oxidative stress. Forty-eight castrated male piglets (10.6 ± 0.99 kg) were fed the following experimental diets: Cont- (low-fat diet, no supplement), Cont+ (high linseed oil diet, no supplement), Vit-E (as Cont+, 105 IU vitamin E/day), OLE-1 (as Cont+, 3.84 mg hydroxytyrosol equivalents (HEQs)/day), OLE-2 (as Cont+, 38.4 mg HEQ/day), and OLE-3 (as Cont+, 96 mg HEQ/day). After 21 days of feeding, the experimental diets, blood and urine samples were collected to assess the extent of the oxidative stress. Results indicated that diet OLE-1 lowered the activity of gamma-glutamyl transferase, protected DNA (measured as DNA tail %) and altered urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG). Dietary vitamin E lowered the levels of urinary F
-isoprostanes, as well as of plasma malondialdehyde and γ-tocopherol, but raised the plasmatic α-tocopherol and altered the level of urinary 8-OHdG. In conclusion, only minor positive effects of dietary OLE on the oxidative stress parameters were observed. Additionally, OLE did not show concentration dependence.