Biosynthesis of palladium nanoparticles by using Moringa oleifera flower extract and their catalytic and biological properties

• Published in: Journal of photochemistry and photobiology. B, Biology Vol. 165; pp. 87 - 95
• Main Authors: Anand, K., Tiloke, C., Phulukdaree, A., Ranjan, B., Chuturgoon, A., Singh, S., Gengan, R.M.
• Format: Journal Article
• Language: English
• Published: Switzerland Elsevier B.V 01-12-2016; Elsevier BV
• Subjects: A549 cells; Acetic acid; Antibacterial activity; Biodegradation; Biological properties; Biomass; Biosynthesis; Biphenyl; Catalysis; Catalysts; Cell Line; Chemical composition; Chemical synthesis; Cytotoxicity; Degradation; Flowers - chemistry; Humans; In vitro methods and tests; Lung carcinoma; Lymphocytes; Metal Nanoparticles; Methylene blue; Microscopy, Electron, Scanning; Microscopy, Electron, Transmission; Moringa oleifera; Moringa oleifera - chemistry; Nanoparticles; Nitrophenol; p-Nitrophenol; Palladium; Palladium - chemistry; Particle Size; PdNPs; Plant Extracts - chemistry; Saline water; Scanning electron microscopy; Spectrophotometry, Ultraviolet; Spectroscopy; Spectroscopy, Fourier Transform Infrared; Spectrum analysis; Studies; Suzuki reaction; Toxicity; Tumor cell lines; Tumors; PdNPs; p-Nitrophenol; Moringa oleifera; A549 cells; Suzuki reaction
• ISSN: 1011-1344; 1873-2682
• DOI: 10.1016/j.jphotobiol.2016.09.039

The biosynthesis of nanostructured biopalladium nanoparticles (PdNPs) from an aqueous solution of crystalline palladium acetate is reported. For the synthesised PdNPs in solution, an agroforest biomass waste petal of Moringa oleifera derived bis-phthalate was used as natural reducing and biocapping agents. Continuous absorption in the UV region and subsequent brown colour change confirmed the formation of PdNPs. A strong surface plasmon peak for PdNPs occurred at 460nm. PdNPs were characterized by SEM with EDX, FTIR, TEM and DLS. The chemical composition of the aqueous extract was determined by GC–MS coupled with FTIR and 1NMR. The catalytic degradation effect by PdNPs on industrial organic toxic effluents p-nitrophenol (PNP) and methylene blue dye was monitored by UV Spectroscopy. On the other hand PdNPs catalysed the base mediated suzuki coupling reaction for biphenyl synthesis, in water. Moreover, PdNPs were found to be reusable catalysts. Toxicity studies of PdNPs showed that the death of brine shrimp to be <50%. Therefore, PdNPs displayed potential for further anticancer studies via tumour cell lines. The in vitro cytotoxicity evaluation of the extract capped nanoparticles was carried out using human lung carcinoma cells (A549) and peripheral lymphocytes normal cells by MTT cell viability assay. Also, PdNPs showed antibacterial activity against Enterococcus faecalis among the different tested strains, including Bacillus cereus, Staphylococcus aureus, Esherichia coli and Candida albicans, Candida utilis.
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•Phytochemical rich flower of Moringa oleifera mediated biosynthesis•PdNPs can reduce methylene blue and p-nitrophenol•Suzuki cross-coupling reaction in water•PdNPs shows antimicrobial, antioxidant and it is nontoxic•Cytotoxic to A549 cells and did not induce toxicity in normal healthy PLs