Deposition of bacteriorhodopsin protein in a purple membrane form on nitrocellulose membranes for enhanced photoelectric response

• Published in: Sensors (Basel, Switzerland) Vol. 13; no. 1; pp. 455 - 462
• Main Authors: Kim, Young Jun, Neuzil, Pavel, Nam, Chang-Hoon, Engelhard, Martin
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 27-12-2012; MDPI
• Subjects: Adenosine; bacteriorhodopsin (bR); Bacteriorhodopsins - metabolism; Collodion - metabolism; Electric currents; Electric Impedance; Electrochemistry - methods; Electrodes; Halobacterium salinarum - metabolism; Hydrogen-Ion Concentration; indium tin oxide (ITO); Indium tin oxides; Light; Photochemistry - methods; Photovoltaic cells; positively charged nitrocellulose membrane (PNM); Proteins; Protons; purple membrane (PM); Purple Membrane - metabolism; Purple Membrane - radiation effects; Sensors; Europe; Germany
• ISSN: 1424-8220; 1424-8220
• DOI: 10.3390/s130100455

Bacteriorhodopsin protein (bR)-based systems are one of the simplest known biological energy converters. The robust chemical, thermal and electrochemical properties of bR have made it an attractive material for photoelectric devices. This study demonstrates the photoelectric response of a dry bR layer deposited on a nitrocellulose membrane with indium tin oxide (ITO) electrodes. Light-induced electrical current as well as potential and impedance changes of dried bR film were recorded as the function of illumination. We have also tested bR in solution and found that the electrical properties are strongly dependent on light intensity changing locally proton concentration and thus pH of the solution. Experimental data support the assumption that bR protein on a positively charged nitrocellulose membrane (PNM) can be used as highly sensitive photo- and pH detector. Here the bR layer facilitates proton translocation and acts as an ultrafast optoelectric signal transducer. It is therefore useful in applications related to bioelectronics, biosensors, bio-optics devices and current carrying junction devices.