Bacterial Cellulose-Modified Polyhydroxyalkanoates Scaffolds Promotes Bone Formation in Critical Size Calvarial Defects in Mice

Bone regeneration is a claim challenge in addressing bone defects with large tissue deficits, that involves bone grafts to support the activity. In vitro biocompatibility of the bacterial cellulose-modified polyhydroxyalkanoates (PHB/BC) scaffolds and its osteogenic potential in critical-size mouse...

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Bibliographic Details
Published in:	Materials Vol. 13; no. 6; p. 1433
Main Authors:	Codreanu, Ada, Balta, Cornel, Herman, Hildegard, Cotoraci, Coralia, Mihali, Ciprian Valentin, Zurbau, Nicoleta, Zaharia, Catalin, Rapa, Maria, Stanescu, Paul, Radu, Ionut-Cristian, Vasile, Eugeniu, Lupu, George, Galateanu, Bianca, Hermenean, Anca
Format:	Journal Article
Language:	English
Published:	Switzerland MDPI AG 21-03-2020 MDPI
Subjects:	bacterial cellulose Biocompatibility Biomedical materials bone tissue engineering Bones Cell adhesion & migration Cellulose Defects Glucose Histology Immunofluorescence Implantation in vivo tests Investigations Laboratories Medicine Polyhydroxyalkanoates R&D Regeneration (physiology) Research & development Scaffolds Skin & tissue grafts Substitute bone Surgical implants Tissue engineering X ray analysis United States > US Switzerland Germany in vivo tests polyhydroxyalkanoates bone tissue engineering bacterial cellulose
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Summary:	Bone regeneration is a claim challenge in addressing bone defects with large tissue deficits, that involves bone grafts to support the activity. In vitro biocompatibility of the bacterial cellulose-modified polyhydroxyalkanoates (PHB/BC) scaffolds and its osteogenic potential in critical-size mouse calvaria defects had been investigated. Bone promotion and mineralization were analyzed by biochemistry, histology/histomorphometry, X-ray analysis and immunofluorescence for highlighting osteogenesis markers. In summary, our results showed that PHB/BC scaffolds are able to support 3T3-L1 preadipocytes proliferation and had a positive effect on in vivo osteoblast differentiation, consequently inducing new bone formation after 20 weeks post-implantation. Thus, the newly developed PHB/BC scaffolds could turn out to be suitable biomaterials for the bone tissue engineering purpose.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Authors with equal contribution.
ISSN:	1996-1944 1996-1944
DOI:	10.3390/ma13061433