Effects of Three-Month Administration of High-Saturated Fat Diet and High-Polyunsaturated Fat Diets with Different Linoleic Acid (LA, C18:2n-6) to α-Linolenic Acid (ALA, C18:3n-3) Ratio on the Mouse Liver Proteome

• Published in: Nutrients Vol. 13; no. 5; p. 1678
• Main Authors: Liput, Kamila P, Lepczyński, Adam, Nawrocka, Agata, Poławska, Ewa, Ogłuszka, Magdalena, Jończy, Aneta, Grzybek, Weronika, Liput, Michał, Szostak, Agnieszka, Urbański, Paweł, Roszczyk, Agnieszka, Pareek, Chandra S, Pierzchała, Mariusz
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 15-05-2021; MDPI
• Subjects: 2DE; alpha-Linolenic Acid - metabolism; Animals; Body Weight; Breast cancer; Carbohydrate metabolism; Carbohydrates; Cardiovascular disease; Cholesterol; Diet; Diet, High-Fat - adverse effects; Electrophoresis; Fatty acids; Fatty Acids, Omega-6; Fatty Acids, Unsaturated - metabolism; Feeds; Gel electrophoresis; Glutathione transferase; Glycerol-3-phosphate dehydrogenase; HFD; High fat diet; Homogenization; Hypertension; Laboratory animals; Linoleic acid; Linoleic Acid - metabolism; Linolenic acid; Liver; Liver - metabolism; Male; Metabolism; Mice; Mortality; nutriproteomics; Oil consumption; Oils & fats; omega-3 PUFA; omega-6 PUFA; Plasma; Proteins; Proteome - metabolism; Proteomes; Proteomics; SFA; Vegetable oils; United States > US; Germany; 2DE; nutriproteomics; HFD; omega-6 PUFA; omega-3 PUFA; MALDI TOF/TOF; SFA
• ISSN: 2072-6643; 2072-6643
• DOI: 10.3390/nu13051678

The aim of the study was to evaluate the effect of different types of high-fat diets (HFDs) on the proteomic profile of mouse liver. The analysis included four dietary groups of mice fed a standard diet (STD group), a high-fat diet rich in SFAs (SFA group), and high-fat diets dominated by PUFAs with linoleic acid (LA, C18:2n-6) to α-linolenic acid (ALA, C18:3n-3) ratios of 14:1 (14:1 group) and 5:1 (5:1 group). After three months of diets, liver proteins were resolved by two-dimensional gel electrophoresis (2DE) using 17 cm non-linear 3-10 pH gradient strips. Protein spots with different expression were identified by MALDI-TOF/TOF. The expression of 13 liver proteins was changed in the SFA group compared to the STD group (↓: ALB, APOA1, IVD, MAT1A, OAT and PHB; ↑: ALDH1L1, UniProtKB-Q91V76, GALK1, GPD1, HMGCS2, KHK and TKFC). Eleven proteins with altered expression were recorded in the 14:1 group compared to the SFA group (↓: ARG1, FTL1, GPD1, HGD, HMGCS2 and MAT1A; ↑: APOA1, CA3, GLO1, HDHD3 and IVD). The expression of 11 proteins was altered in the 5:1 group compared to the SFA group (↓: ATP5F1B, FTL1, GALK1, HGD, HSPA9, HSPD1, PC and TKFC; ↑: ACAT2, CA3 and GSTP1). High-PUFA diets significantly affected the expression of proteins involved in, e.g., carbohydrate metabolism, and had varying effects on plasma total cholesterol and glucose levels. The outcomes of this study revealed crucial liver proteins affected by different high-fat diets.