Anti-Inflammatory Effects of Angelica sinensis (Oliv.) Diels Water Extract on RAW 264.7 Induced with Lipopolysaccharide

• Published in: Nutrients Vol. 10; no. 5; p. 647
• Main Authors: Kim, Young-Jin, Lee, Ji Young, Kim, Hyun-Ju, Kim, Do-Hoon, Lee, Tae Hee, Kang, Mi Suk, Park, Wansu
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 21-05-2018; MDPI
• Subjects: Angelica sinensis; anti-inflammatory; Apoptosis; Assaying; c-Fos protein; Calcium; Calcium (intracellular); Cardiovascular diseases; Cell activation; CHOP protein; Colonies; Colony-stimulating factor; Colorimetry; Cyclooxygenase-2; cytokine; Gene expression; Ginseng; Granulocyte colony-stimulating factor; Granulocyte-macrophage colony-stimulating factor; Granulocytes; Herbal medicine; Inflammation; Interleukin 10; JAK-STAT; Janus kinase; Janus kinase 2; Kinases; Lipopolysaccharides; Lymphocytes T; macrophage; Macrophage inflammatory protein; Macrophages; Molecular chains; Monocytes; Nitric oxide; Polymerase chain reaction; Proteins; Reagents; Rodents; Transcription factors; Transducers; Tumor necrosis factor-TNF; Vascular endothelial growth factor; cytokine; nitric oxide; calcium; JAK-STAT; anti-inflammatory; macrophage; Angelica sinensis
• ISSN: 2072-6643; 2072-6643
• DOI: 10.3390/nu10050647

The dry root of
(Oliv.) Diels, also known as "female ginseng", is a popular herbal drug amongst women, used to treat a variety of health issues and cardiovascular diseases. The aim of this study is to evaluate the detailed molecular mechanism for anti-inflammatory effects of
root water extract (ASW). The anti-inflammatory effect of ASW on lipopolysaccharide (LPS)-induced RAW 264.7 mouse macrophages was evaluated by the tetrazolium-based colorimetric assay (MTT), Griess reagent assay, multiplex cytokine assay, real time reverse transcription polymerase chain reaction (RT-PCR), and Fluo-4 calcium assay. ASW restored cell viability in RAW 264.7 at concentrations of up to 200 µg/mL. ASW showed notable anti-inflammatory effects. ASW exhibited IC
= 954.3, 387.3, 191.7, 317.8, 1267.0, 347.0, 110.1, 573.6, 1171.0, 732.6, 980.8, 125.0, and 257.0 µg/mL for interleukin (IL)-6, tumor necrosis factor (TNF)-α, monocyte chemotactic activating factor (MCP)-1, regulated on activation, normal T cell expressed and secreted (RANTES), granulocyte colony-stimulating factor (G-CSF), granulocyte macrophage colony-stimulating factor (GM-CSF), vascular endothelial growth factor (VEGF), lipopolysaccharide-induced CXC chemokine (LIX), macrophage inflammatory protein (MIP)-1α, MIP-1β, MIP-2, IL-10, and intracellular calcium, respectively. Additionally, ASW inhibited the LPS-induced production of nitric oxide and the LPS-induced mRNA expression of CHOP (GADD153), Janus kinase 2 (JAK2), signal transducers and activators of transcription 1 (STAT1), first apoptosis signal receptor (FAS), and c-Fos, NOS2, and PTGS2 (COX2) in RAW 264.7 significantly (
< 0.05). Data suggest that ASW exerts an anti-inflammatory effect on LPS-induced RAW 264.7 via NO-bursting/calcium-mediated JAK-STAT pathway.