The Photodynamic Properties and the Genotoxicity of Heat-Treated Silicalite-1 Films

• Published in: Materials Vol. 12; no. 4; p. 567
• Main Authors: Jirka, Ivan, Kopová, Ivana, Kubát, Pavel, Tabor, Edyta, Bačáková, Lucie, Bouša, Milan, Sajdl, Petr
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 14-02-2019; MDPI
• Subjects: Alloy steels; Alloys; Austenitic stainless steels; Bacteria; Biocompatibility; Biomedical materials; Chemical synthesis; Chromatography; Cytotoxicity; Deoxyribonucleic acid; DNA; Fourier transforms; Gas chromatography; Genotoxicity; Heat; Heat treatment; implant material; Infrared analysis; Infrared spectroscopy; Mass spectrometry; Metabolism; Metabolites; Metal foils; Mutation; Photoelectrons; Prostheses; Silicalite; singlet oxygen; Slurries; Stainless steel; surface coating; Titanium base alloys; Transplants & implants; X ray photoelectron spectroscopy; Zeolites; genotoxicity; surface coating; singlet oxygen; implant material
• ISSN: 1996-1944; 1996-1944
• DOI: 10.3390/ma12040567

We investigated the use of a supported silicalite-1 film (
) as a promising coating for metallic materials used in the fabrication of prostheses. The role of carbonaceous residua present on high-temperature calcined-
in generating singlet oxygen for future use as a sterilization method has also been addressed, and the potential genotoxicity of these residua in osteoblast-like cells has been investigated. Calcination of as-synthesized
induced the appearance of a rather complicated mixture of aliphatic and aromatic species on its outer surface. A series of variously volatile polycyclic aromatic hydrocarbons (PAH), including naphthalene, fluorene, phenanthrene, anthracene, fluoranthene, and pyrene, were identified in micromole concentrations. Irradiation of these PAHs on calcined-
immersed in air-saturated chloroform led to the formation of very low concentrations of singlet oxygen. However, an increased level of DNA damage was observed on calcined-SF by immunofluorescence staining of phosphorylated histone H2AX analyzed by flow cytometry.