Cornus officinalis Ethanolic Extract with Potential Anti-Allergic, Anti-Inflammatory, and Antioxidant Activities

• Published in: Nutrients Vol. 12; no. 11; p. 3317
• Main Authors: Quah, Yixian, Lee, Seung-Jin, Lee, Eon-Bee, Birhanu, Biruk Tesfaye, Ali, Md Sekendar, Abbas, Muhammad Aleem, Boby, Naila, Im, Zi-Eum, Park, Seung-Chun
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 29-10-2020; MDPI
• Subjects: Animals; Anti-Allergic Agents - pharmacology; anti-inflammatory activity; Anti-Inflammatory Agents - pharmacology; antioxidant activity; Antioxidants; Antioxidants - pharmacology; Apoptosis; Atopic dermatitis; Attenuation; Chromatography; Chromatography, Liquid; Cornus; Cornus officinalis; Cytokines; Cytokines - metabolism; Cytotoxicity; Dermatitis; Eczema; Ethanol - pharmacology; Fc receptors; Free radicals; Gas chromatography; Gene expression; human high-affinity IgE receptors; Human serum albumin; Humans; Hypersensitivity; IL-1β; Immunoglobulin E; Interleukin 6; Lipopolysaccharides; Lipopolysaccharides - metabolism; Liquid chromatography; Mass spectrometry; Mass spectroscopy; Mice; Molecular docking; Molecular Docking Simulation; Naringenin; NF-κB protein; Nitric oxide; Nitric Oxide - biosynthesis; Nitric-oxide synthase; Oxidative stress; Oxidative Stress - drug effects; Plant Extracts - pharmacology; Proteins; RAW 264.7 Cells; Scavenging; Scientific imaging; Serum albumin; Skin diseases; Spectroscopy; Sulfonic acid; Tandem Mass Spectrometry; Tumor necrosis factor-α; United States > US; Japan; atopic dermatitis; human high-affinity IgE receptors; molecular docking; Cornus officinalis; antioxidant activity; anti-inflammatory activity
• ISSN: 2072-6643; 2072-6643
• DOI: 10.3390/nu12113317

Atopic dermatitis (AD) is an allergic and chronic inflammatory skin disease. The present study investigates the anti-allergic, antioxidant, and anti-inflammatory activities of the ethanolic extract of
(COFE) for possible applications in the treatment of AD. COFE inhibits the release of β-hexosaminidase from RBL-2H3 cells sensitized with the dinitrophenyl-immunoglobulin E (IgE-DNP) antibody after stimulation with dinitrophenyl-human serum albumin (DNP-HSA) in a concentration-dependent manner (IC
= 0.178 mg/mL). Antioxidant activity determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, ferric reducing antioxidant power assay, and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) scavenging activity, result in EC
values of 1.82, 10.76, and 0.6 mg/mL, respectively. Moreover, the extract significantly inhibits lipopolysaccharide (LPS)-induced nitric oxide (NO) production and the mRNA expression of iNOS and pro-inflammatory cytokines (IL-1β, IL-6, and TNF-α) through attenuation of NF-κB activation in RAW 264.7 cells. COFE significantly inhibits TNF-α-induced apoptosis in HaCaT cells without cytotoxic effects (
< 0.05). Furthermore, 2-furancarboxaldehyde and loganin are identified by gas chromatography/mass spectrometry (GC-MS) and liquid chromatography with tandem mass spectrometry (LC-MS/MS) analysis, respectively, as the major compounds. Molecular docking analysis shows that loganin, cornuside, and naringenin 7-O-β-D-glucoside could potentially disrupt the binding of IgE to human high-affinity IgE receptors (FceRI). Our results suggest that COFE might possess potential inhibitory effects on allergic responses, oxidative stress, and inflammatory responses.