Cellular Integration of an Enzyme-Loaded Polymersome Nanoreactor

Cellular Integration of an Enzyme-Loaded Polymersome Nanoreactor

Cells with implants: Porous enzyme‐loaded polymersomes were constructed that display the cell‐penetrating peptide tat on their surfaces. These nanoreactors are taken up by mammalian cells through macropinocytosis. Inside the cells, the polymersomes are only partially routed to acidic compartments. P...

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Published in:Angewandte Chemie (International ed.) Vol. 49; no. 40; pp. 7213 - 7216
Main Authors: van Dongen, Stijn F.M, Verdurmen, Wouter P.R, Peters, Ruud J.R.W, Nolte, Roeland J.M, Brock, Roland, van Hest, Jan C.M
Format: Journal Article
Language:English
Published: Weinheim Wiley-VCH Verlag 24-09-2010
WILEY-VCH Verlag
WILEY‐VCH Verlag
Subjects:
bioorganic chemistry
Bioreactors
cell-penetrating peptides
Dextrans - chemistry
Flow Cytometry
HeLa Cells
Humans
Microscopy, Confocal
Microscopy, Fluorescence
Molecular Structure
Nanostructures - chemistry
nanotechnology
Polymers - chemistry
polymersomes
Porosity
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Summary:Cells with implants: Porous enzyme‐loaded polymersomes were constructed that display the cell‐penetrating peptide tat on their surfaces. These nanoreactors are taken up by mammalian cells through macropinocytosis. Inside the cells, the polymersomes are only partially routed to acidic compartments. Polymersomes with horseradish peroxidase as a model cargo enzyme displayed sustained intracellular activity.
Bibliography:http://dx.doi.org/10.1002/anie.201002655
Netherlands Research School Combination-Catalysis (NRSC-C)
istex:157FC51045A954ED376A05D13BF61F433F99B631
ArticleID:ANIE201002655
ark:/67375/WNG-Q8HFPSWQ-1
We thank Dr. E. Pierson for technical assistance and Hans-Peter de Hoog and Morten B. Hansen for synthesis of PS-PIAT and azido-tat, respectively. S.F.M.v.D. acknowledges the Netherlands Research School Combination-Catalysis (NRSC-C) for financial support. W.P.R.V. was supported by the UMC internal funding programme.
These authors contributed equally to this work.
We thank Dr. E. Pierson for technical assistance and Hans‐Peter de Hoog and Morten B. Hansen for synthesis of PS‐PIAT and azido‐tat, respectively. S.F.M.v.D. acknowledges the Netherlands Research School Combination—Catalysis (NRSC‐C) for financial support. W.P.R.V. was supported by the UMC internal funding programme.
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SourceType-Scholarly Journals-1
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ISSN:1433-7851
1521-3773
DOI:10.1002/anie.201002655