Genetic mapping of adult plant leaf rust resistance genes Lr48 and Lr49 in common wheat

• Published in: Theoretical and applied genetics Vol. 117; no. 3; pp. 307 - 312
• Main Authors: Bansal, U. K, Hayden, M. J, Venkata, B. P, Khanna, R, Saini, R. G, Bariana, H. S
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Berlin/Heidelberg : Springer-Verlag 01-08-2008; Springer-Verlag; Springer; Springer Nature B.V
• Subjects: Agriculture; Alleles; Biochemistry; Biological and medical sciences; Biomedical and Life Sciences; Biotechnology; Chromosome Mapping; Chromosome Segregation; Classical genetics, quantitative genetics, hybrids; Fundamental and applied biological sciences. Psychology; Genes; Genes, Plant; Genetic Linkage; Genetics of eukaryotes. Biological and molecular evolution; Genomes; Immunity, Innate - genetics; Inbreeding; Infections; Inheritance Patterns - genetics; Life Sciences; Methods, theories and miscellaneous; Microsatellite Repeats - genetics; Original Paper; Plant Biochemistry; Plant Breeding/Biotechnology; Plant Diseases - genetics; Plant Diseases - immunology; Plant Genetics and Genomics; Plant Leaves - genetics; Plant resistance; Polymerase Chain Reaction; Pteridophyta, spermatophyta; Triticum - genetics; Vegetals; Australia; India; Leaf Rust Resistance Gene; Adult Plant Resistance; Leaf Rust; Recombinant Inbred Line Population; Stem Rust Resistance; Human; Genetic mapping; Monocotyledones; Plant leaf; Resistance; Gene; Gramineae; Angiospermae; Adult; Genetics; Spermatophyta; Agriculture; Triticum aestivum
• ISSN: 0040-5752; 1432-2242
• DOI: 10.1007/s00122-008-0775-6

Hypersensitive adult plant resistance genes Lr48 and Lr49 were named based on their genetic independence of the known adult plant resistance genes. This study was planned to determine genomic locations of these genes. Recombinant inbred line populations derived from crosses involving CSP44 and VL404, sources of Lr48 and Lr49, respectively, and the susceptible parent WL711, were used to determine the genomic locations of these genes. Bulked segregant analyses were performed using multiplex-ready PCR technology. Lr48 in genotype CSP44 was mapped on chromosome arm 2BS flanked by marker loci Xgwm429b (6.1 cM) and Xbarc7 (7.3 cM) distally and proximally, respectively. Leaf rust resistance gene Lr13, carried by the alternate parent WL711, was proximal to Lr48 and was flanked by Xksm58 (5.1 cM) and Xstm773-2 (8.7 cM). Lr49 was flanked by Xbarc163 (8.1 cM) and Xwmc349 (10.1 cM) on chromosome arm 4BL. The likely presence of the durable leaf rust resistance gene Lr34 in both CSP44 and VL404 was confirmed using the tightly linked marker csLV34. Near-isogenic lines for Lr48 and Lr49 were developed in cultivar Lal Bahadur. Genotypes combining Lr13 and/or Lr34 with Lr48 or Lr49 were identified as potential donor sources for cultivar development programs.