Removal of nitrogen by heterotrophic nitrification-aerobic denitrification of a novel metal resistant bacterium Cupriavidus sp. S1

• Published in: Bioresource technology Vol. 220; pp. 142 - 150
• Main Authors: Sun, Zhiyi, Lv, Yongkang, Liu, Yuxiang, Ren, Ruipeng
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01-11-2016
• Subjects: Aerobic denitrification; Aerobiosis; Ammonium Compounds - isolation & purification; Cupriavidus - metabolism; Cupriavidus sp. S1; Denitrification; Heavy metal; Heterotrophic nitrification; Heterotrophic Processes; Metals; Nitrates - isolation & purification; Nitrification; Nitrites - isolation & purification; Nitrogen - isolation & purification; Nitrogen - metabolism; Nitrogen removal; Waste Water; Nitrogen removal; Heterotrophic nitrification; Cupriavidus sp. S1; Aerobic denitrification; Heavy metal
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2016.07.110

•A new heterotrophic nitrifying and metal resistant bacterium, named S1, was isolated and identified as Cupriavidus sp.•Strain S1 possessed excellent heterotrophic nitrification and aerobic denitrification ability.•It could utilize different forms of nitrogen source in simultaneous nitrification and denitrification.•NH4+ could effectually remove with high concentration heavy metal involved in.
A novel heterotrophic nitrifying and metal resistant bacterium was isolated and identified as Cupriavidus sp. S1. The utilization of ammonium, nitrate and nitrite as well as the production of N2 proved the heterotrophic nitrification and aerobic denitrification ability of S1. The ammonium, nitrate and nitrite removal efficiencies were 99.68%, 98.03% and 99.81%, with removal rates of 10.43, 8.64 and 8.36mg/L/h, respectively. A multiple regression equation well described the relationship between carbon source utilization, cell growth and nitrification. Keeping the shaking speed at 120rpm was beneficial for denitrification. Moreover, different forms of nitrogen source could be utilize in simultaneous nitrification and denitrification. Additionally, the efficient removal of ammonium occurred at 20.0mg/LZn2+, or 10.0mg/LNi2+ or 8.0mg/LCu2+ or 5.0mg/LCr6+, 33.35mmol/L sodium pyruvate, C/N 12–28. These findings demonstrate that S1 was effective for nitrogen removal in industrial wastewater containing heavy metal.