GC/MS-Based Metabolomics Approach to Evaluate the Effect of Jackyakgamcho-Tang on Acute Colitis

• Published in: Evidence-based complementary and alternative medicine Vol. 2019; no. 2019; pp. 1 - 10
• Main Authors: Seo, Chang-Seob, Jun, Ki Young, Kim, Dae Sung, Na, Changsu, Son, Hong-Seok, Park, Dae-Hun, Bok, So-Hyeon, Lee, Soon-Young, Lee, Yu-Mi, Youn, Daehwan, Kim, Eun-Ju, Park, Seong-Eun, Seo, Seung-Ho, Byun, Sung-Hoon
• Format: Journal Article
• Language: English
• Published: Cairo, Egypt Hindawi Publishing Corporation 01-01-2019; Hindawi; Hindawi Limited
• Subjects: Apoptosis; Chemical compounds; Colitis; Cytokines; Deoxyribonucleic acid; DNA; Evidence-based medicine; Fatty acids; Feces; Herbal medicine; Homeostasis; Inflammation; Inflammatory bowel disease; Interleukin 6; Intestinal microflora; Laboratories; Lactic acid; Linoleic acid; Metabolites; Metabolomics; Microbiota; Natural products; Nutrition; Pain; Tumor necrosis factor-α; United States > US; Japan
• ISSN: 1741-427X; 1741-4288
• DOI: 10.1155/2019/4572764

The objective of this study was to examine the effects of Jackyakgamcho-tang (JGT) on acute colitis. GC/MS-based metabolomics and NGS-based metagenomics were applied to investigate the alteration of metabolites and microbiota in an acute colitis model. The severity of acute colitis symptoms was alleviated by JGT treatment. Induction of colitis and JGT treatment changed compositions of gut microbiota and inflammatory cytokine levels (TNF-α and IL-6). They also substantially change metabolites (i.e., lactic acid, linoleic acid, monostearin, and palmitoylglycerol). In addition, some clear correlations were observed among metabolites, cytokine, and microbiota. This study highlights the applicability of metabolomics and metagenomics study for evaluating anti-inflammatory effects of a new functional herbal medicine as a therapeutic agent for acute colitis.