Development of a Novel Silver Nanoparticles-Enhanced Screen-Printed Amperometric Glucose Biosensor

• Published in: Analytical letters Vol. 41; no. 7; pp. 1158 - 1172
• Main Authors: Zuo, Shaohua, Teng, Yuanjie, Yuan, Huihui, Lan, Minbo
• Format: Journal Article
• Language: English
• Published: Philadelphia, PA Taylor & Francis Group 01-01-2008; Taylor & Francis
• Subjects: Amperometric biosensor; Analytical chemistry; Biological and medical sciences; Biosensors; Biotechnology; Chemistry; Electrochemical methods; Exact sciences and technology; Fundamental and applied biological sciences. Psychology; General, instrumentation; Glucose oxidase; Methods. Procedures. Technologies; Prussian blue; Screen-printed electrode; Silver nanoparticles; Various methods and equipments; Phosphates; Glucose oxidase; Dyes; Hexacyanoferrates; Nanoparticle; Immobilization; Rabbit; Alcohol; Amperometric biosensor; Blood; Amperometry; Serum; Silica gel; Prussian blue; Silver nanoparticles; Disposable; Enzyme; Lagomorpha; Chemical sensor; Screen- printed electrode; Silver; Vertebrata; Sensitivity; Mammalia; Sol gel process; Biosensor; Oxidoreductases
• ISSN: 0003-2719; 1532-236X
• DOI: 10.1080/00032710802052361

A disposable glucose biosensor is developed by immobilizing glucose oxidase into silver nanoparticles-doped silica sol-gel and polyvinyl alcohol hybrid film on a Prussian blue-modified screen-printed electrode. The silver nanoparticles-enhanced biosensor shows a linear amperometric response to glucose from 1.25 × 10
−5
to 2.56 × 10
−3
with a sensitivity of 20.09 mA M
−1
 cm
−2
, which is almost double that of the biosensors without silver nanoparticles. The immobilized glucose oxidase retained 91% of its original activity after 30 days of storage in phosphate buffer (pH 6.9; 0.1 M) at 4°C. Blood glucose in a rabbit serum sample was successfully measured with the biosensor.