Reprogramming Enhancers in Somatic Cell Nuclear Transfer, iPSC Technology, and Direct Conversion

• Published in: Stem cell reviews Vol. 13; no. 1; pp. 24 - 34
• Main Authors: Kwon, Daekee, Ji, Minjun, Lee, Seunghee, Seo, Kwang Won, Kang, Kyung-Sun
• Format: Journal Article
• Language: English
• Published: New York Springer US 01-02-2017
• Subjects: Animals; Biomedical and Life Sciences; Biomedical Engineering and Bioengineering; Cell Biology; Cell Culture Techniques - methods; Cell Differentiation; Cellular Reprogramming; Humans; Induced Pluripotent Stem Cells - cytology; Induced Pluripotent Stem Cells - metabolism; Life Sciences; Nuclear Transfer Techniques; Regenerative Medicine - methods; Regenerative Medicine - trends; Regenerative Medicine/Tissue Engineering; Reproducibility of Results; Stem Cells; Reprogramming; Induced pluripotent stem cells; Somatic cell nuclear transfer; Reprogramming enhancer; Reprogramming efficiency; Direct conversion
• ISSN: 1550-8943; 1558-6804; 2629-3277
• DOI: 10.1007/s12015-016-9697-x

Direct conversion of cell fate is a core technology for future regenerative medicine. However, the low reprogramming efficiency and low reproducibility are the largest obstacles blocking translational research of direct conversion technology. This review lists the major reprogramming enhancers verified in the representative reprogramming technologies of somatic cell nuclear transfer, induced pluripotent stem cell technology, and direct conversion, and categorizes them according to their functions. Ultimately, we strive to provide a practical plan to overcome the low reprogramming efficiency and low reproducibility of direct conversion.