Expression of Nestin and Glial Fibrillary Acidic Protein in the Marginal Ischemic Zone of the Brain in SHR Rats

• Published in: Bulletin of experimental biology and medicine Vol. 169; no. 4; pp. 576 - 581
• Main Authors: Tsyba, D. L., Kirik, O. V., Kolpakova, M. E., Yakovleva, A. A., Korzhevskii, D. E.
• Format: Journal Article
• Language: English
• Published: New York Springer US 01-08-2020; Springer
• Subjects: Biomedical and Life Sciences; Biomedicine; Brain; Cell Biology; Cytochemistry; Hypertension; Intermediate filament proteins; Internal Medicine; Ischemia; Laboratory Medicine; Pathology; Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine); focal cerebral ischemia; glial fibrillary acidic protein; neural progenitor cells; nestin
• ISSN: 0007-4888; 1573-8221
• DOI: 10.1007/s10517-020-04932-4

We studied spatial organization and structural characteristics of striatal glial cells in spontaneously hypertensive rats (SHR) in 48 h after 30-min focal ischemia. Immunocytochemical analysis of nestin and glial fibrillar acidic protein (GFAP) revealed 3 types of activated astrocytes: expressing only nestin, only GFAP, or both markers. There were no nestin-immunopositive astrocytes in the striatum of sham-operated rats. In cells expressing nestin and GFAP, localization of these markers did not completely coincide, which can be explained by different functions of these proteins or formation of heterodimers of nestin with other intermediate filament proteins.