Quality of placental RNA: Effects of explant size and culture duration

Abstract We evaluated the impact of placental micro (≤50 mg) and macro (∼200 mg) explants, oxygen concentration and culture method on placental RNA quality after long-term culture. Our findings show that micro explants cultured at 8% oxygen have the best RNA quality and tissue structure. Macro expla...

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Bibliographic Details
Published in:	Placenta (Eastbourne) Vol. 46; pp. 45 - 48
Main Authors:	Brew, O, Nikolopoulou, E, Hughes, A, Christian, M, Lee, Y, Oduwole, O, Sullivan, M.H.F, Woodman, A
Format:	Journal Article
Language:	English
Published:	Netherlands Elsevier Ltd 01-10-2016
Subjects:	Explant viability Female Humans Internal Medicine Macro explants Micro explant Obstetrics and Gynecology Oxygen concentration Placenta Placenta - metabolism Pregnancy RNA - metabolism RNA quality Syncytial regeneration Tissue Culture Techniques Oxygen concentration Placenta Macro explants Syncytial regeneration Explant viability Micro explant RNA quality
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Summary:	Abstract We evaluated the impact of placental micro (≤50 mg) and macro (∼200 mg) explants, oxygen concentration and culture method on placental RNA quality after long-term culture. Our findings show that micro explants cultured at 8% oxygen have the best RNA quality and tissue structure. Macro explants were less viable after long-term culture. Macro explants and explants undergoing syncytial degeneration produced poor quality RNA and should be avoided.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0143-4004 1532-3102
DOI:	10.1016/j.placenta.2016.08.083