Interleukin 2- and Interleukin 5-Induced Changes in the Binding of Regulatory Factors to the J-Chain Gene Promoter

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 88; no. 24; pp. 11027 - 11031
• Main Authors: MCFADDEN, H. J, KOSHLAND, M. E
• Format: Journal Article
• Language: English
• Published: Washington, DC National Academy of Sciences of the United States of America 15-12-1991; National Acad Sciences
• Subjects: Animals; B lymphocytes; Binding Sites; Biological and medical sciences; Cell Line; Cell lines; Cell nucleus; Cell Nucleus - physiology; Cells; Fundamental and applied biological sciences. Psychology; Gels; Gene expression; Genes; Genes, Immunoglobulin; Humans; Immunoglobulin J-Chains - genetics; Immunoglobulin M - genetics; Interleukin-2 - pharmacology; Interleukin-5 - pharmacology; Interleukins; Lymphoma, B-Cell; Mice; Molecular and cellular biology; Molecular genetics; Nuclear proteins; Nuclear Proteins - metabolism; Plasma cells; Promoter Regions, Genetic - drug effects; Recombinant Proteins - pharmacology; Restriction Mapping; RNA; TATA Box; Transcription, Genetic - drug effects; Immunoglobulins; Immune response; Transcription promoter; Cytokine; Gene expression; J-Peptide chain; Lymphoma; IgM; Immunogenetics; Regulation(control); Binding capacity; Interleukin 5; Gene; Interleukin 2; Trans acting regulatory factor
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.88.24.11027

In a primary immune response, B cells require signals from the T-cell lymphokines interleukins 2 and 5 (IL-2 and IL-5) to develop into IgM-secreting cells. One role of IL-2 and IL-5 is to activate transcription of the gene encoding the IgM joining component, the J chain. In this study the activation mechanism was investigated by using an inducible B-lymphoma cell line to examine J-chain RNA expression and factor binding to the J-chain promoter. The analyses revealed that both IL-2 and IL-5 trigger a decrease in the binding of two promoter-specific nuclear proteins that precedes the appearance of J-chain RNA. In combination the two lymphokines effected nearly additive changes in factor binding and J-chain RNA abundance. Both effects were reversed upon withdrawal of the lymphokine stimulus and both were inhibited in the presence of the T-cell lymphokine IL-4. These findings indicate that the IL-2 and IL-5 signal pathways converge to deliver a common signal that regulates the repressor activities of two lymphokine-responsive promoter elements.