Metabolic profiles and phylogenetic diversity of microbial communities from chlorinated pesticides contaminated sites of different geographical habitats of India

• Published in: Journal of applied microbiology Vol. 109; no. 4; pp. 1458 - 1468
• Main Authors: Manickam, N, Pathak, A, Saini, H.S, Mayilraj, S, Shanker, R
• Format: Journal Article
• Language: English
• Published: Oxford, UK Oxford, UK : Blackwell Publishing Ltd 01-10-2010; Blackwell Publishing Ltd; Blackwell
• Subjects: 16S rRNA phylogeny; Bacteria - classification; Bacteria - genetics; Bacteria - metabolism; Biodegradation, Environmental; Biological and medical sciences; Fundamental and applied biological sciences. Psychology; India; linA-dehydrodechlorinase; Lindane - analysis; Lindane - metabolism; metagenome; Metagenomics; microbial communities; Microbiology; Pesticide Residues - analysis; pesticides; Pesticides - metabolism; Phylogeny; Sequence Alignment; Soil; Soil Microbiology; Soil Pollutants - analysis; Soil Pollutants - metabolism; Sphingomonas; Sphingomonas - genetics; Asia; India; Applied microbiology; Diversity; Pesticides; Phylogeny; microbial communities; Metabolism; Chlorine compounds; metagenome; Ribosomal RNA; linA-dehydrodechlorinase; 16S-RNA; 16S rRNA phylogeny; Microbial community
• ISSN: 1364-5072; 1365-2672
• DOI: 10.1111/j.1365-2672.2010.04781.x

To study the microbial communities in three sites contaminated with chlorinated pesticides and evaluation of dehydrodechlorinase (linA) gene variants involved in gamma-hexachlorocyclohexane (γ-HCH, lindane) degradation. Using a culture-independent method, 16S rRNA genes were amplified from microbial communities occurring in contaminated soils. From 375 clone libraries analysed, 55 different restriction fragment length polymorphism phylotypes were obtained. Dehydrodechlorinase (linA) gene, which initiates the γ-HCH degradation, was directly amplified by PCR from the DNA extracted from soils. Deduced amino acid sequences of eight variant genotypes of linA showed few amino acid changes. All the variants of linA had mutations of F151L and S154T, and one of the genotype carried 12 amino acid changes when compared to a linA of Sphingomonas sp. reported from the same soil. The microbial communities displayed complex and diverse groups similar to bacteria involved in biodegradation. The presence of biodegradative genes like linA indicates the presence of communities with capacity to biodegrade the persistent pesticide HCH. This study provides insights to evaluate the presence of catabolic genes and assessing the bioremediation potential of the industrial soils contaminated by chlorinated pesticides.