Qualitative and quantitative analysis of flavonoids from 12 species of Korean mulberry leaves

• Published in: Journal of food science and technology Vol. 55; no. 5; pp. 1789 - 1796
• Main Authors: Ju, Wan-Taek, Kwon, O-Chul, Kim, Hyun-Bok, Sung, Gyoo-Byung, Kim, Heon-Woong, Kim, Yong-Soon
• Format: Journal Article
• Language: English
• Published: New Delhi Springer India 01-05-2018; Springer Nature B.V
• Subjects: Chemistry; Chemistry and Materials Science; Chemistry/Food Science; Flavonoids; Flavonols; Food Science; Glucosides; Glycosides; Herbal medicine; High performance liquid chromatography; Ions; Kaempferol; Leaves; Liquid chromatography; Mass spectrometry; Mass spectroscopy; Nutrition; Original; Original Article; Quadrupoles; Qualitative analysis; Quantitative analysis; Quercetin; Rutin; Studies; Mulberry leaves; Flavonoids; UPLC–DAD–QTOF/MS; L; Morus alba L
• ISSN: 0022-1155; 0975-8402
• DOI: 10.1007/s13197-018-3093-2

The total flavonoids in leaves of 12 varieties of Korean mulberry (
Morus alba
L.) were determined. Seventeen flavonoids were isolated and analyzed using ultra-performance liquid chromatography coupled with diode array detection and quadrupole time-of-flight mass spectrometry (UPLC–DAD–QTOF/MS). To determine the flavonoid contents, HPLC analysis was performed on these 17 flavonoids. The total flavonoid contents of the 12 varieties of mulberry leaves ranged from 748.5 to 1297.9 mg, with the highest obtained from the Cheong Su variety (1297.9 ± 112.0 mg). Among the 17 flavonoids analyzed, quercetin 3-
O
-rutinoside (rutin) and quercetin 3-
O
-glucoside (isoquercitrin) had highest contents in the Cheong Su variety. Furthermore, the Dae Dang Sang variety gave the highest quercetin 3-
O
-rutinoside (rutin) content among the mulberry leaves investigated, at 425.5 ± 45.9 mg. Major flavonols from Dae Dang Sang were detected by UPLC–DAD–QTOF/MS. A total of 17 flavonoid compound peaks were identified in the analysis time range of 5–40 min, all of which were kaempferol and quercetin glycosides. Seven of the 17 compounds identified in mulberry leaves were unknown.