Development of a new model of actinic enteritis in rats using a cobalt-60 open source and a protection device as a collimator
To describe a new model of actinic enteritis that does not use radiotherapy machines. Sixteen Wistar rats were divided into four groups, consisting of four animals each: control (group A), two weeks after irradiation (group B), five weeks after irradiation (group C) and eight weeks after irradiation...
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| Published in: | Acta cirurgica brasileira Vol. 32; no. 4; pp. 319 - 324 |
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| Format: | Journal Article |
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Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
01-04-2017
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| Abstract | To describe a new model of actinic enteritis that does not use radiotherapy machines.
Sixteen Wistar rats were divided into four groups, consisting of four animals each: control (group A), two weeks after irradiation (group B), five weeks after irradiation (group C) and eight weeks after irradiation (group D). Animals were given a 10Gy radiation from a Cobalt-60 natural source in a nuclear technology research center. Protections of the surrounding tissues were obtained through the usage of plumb devices with a hole in the center, which served as a collimator. We obtained irradiated and non-irradiated colons from each animal.
In group B we found an important inflammatory response in the irradiated colon, which appeared in a reduced way in group C and was minimal in group D, in which we found a relevant collagen submucosal deposition/fibrosis. In all groups, the non-irradiated colon had a lower pathological damage in comparison with the irradiated colon.
We thus described an efficient and feasible technique for obtaining an animal model of actinic enteritis. |
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| AbstractList | To describe a new model of actinic enteritis that does not use radiotherapy machines.
Sixteen Wistar rats were divided into four groups, consisting of four animals each: control (group A), two weeks after irradiation (group B), five weeks after irradiation (group C) and eight weeks after irradiation (group D). Animals were given a 10Gy radiation from a Cobalt-60 natural source in a nuclear technology research center. Protections of the surrounding tissues were obtained through the usage of plumb devices with a hole in the center, which served as a collimator. We obtained irradiated and non-irradiated colons from each animal.
In group B we found an important inflammatory response in the irradiated colon, which appeared in a reduced way in group C and was minimal in group D, in which we found a relevant collagen submucosal deposition/fibrosis. In all groups, the non-irradiated colon had a lower pathological damage in comparison with the irradiated colon.
We thus described an efficient and feasible technique for obtaining an animal model of actinic enteritis. Abstract Purpose: To describe a new model of actinic enteritis that does not use radiotherapy machines. Methods: Sixteen Wistar rats were divided into four groups, consisting of four animals each: control (group A), two weeks after irradiation (group B), five weeks after irradiation (group C) and eight weeks after irradiation (group D). Animals were given a 10Gy radiation from a Cobalt-60 natural source in a nuclear technology research center. Protections of the surrounding tissues were obtained through the usage of plumb devices with a hole in the center, which served as a collimator. We obtained irradiated and non-irradiated colons from each animal. Results: In group B we found an important inflammatory response in the irradiated colon, which appeared in a reduced way in group C and was minimal in group D, in which we found a relevant collagen submucosal deposition/fibrosis. In all groups, the non-irradiated colon had a lower pathological damage in comparison with the irradiated colon. Conclusion: We thus described an efficient and feasible technique for obtaining an animal model of actinic enteritis. |
| Author | Nunes, Vinicius Rodrigues Taranto Barbuto, Rafael Calvão Duval-Araujo, Ivana Pereira, Márcio Tadeu Ladeira, Luís Carlos Duarte Vidigal, Paula Vieira Teixeira |
| Author_xml | – sequence: 1 givenname: Vinicius Rodrigues Taranto surname: Nunes fullname: Nunes, Vinicius Rodrigues Taranto organization: MS, Fellow PhD degree, Medical School, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte-MG, Brazil. Conception and design of the study, acquisition of data, manuscript writing – sequence: 2 givenname: Paula Vieira Teixeira surname: Vidigal fullname: Vidigal, Paula Vieira Teixeira organization: PhD, Assistant Professor, Medical School, UFMG, Belo Horizonte-MG, Brazil. Acquisition of data, critical revision – sequence: 3 givenname: Márcio Tadeu surname: Pereira fullname: Pereira, Márcio Tadeu organization: Nuclear Physicist, PhD, Centro de Desenvolvimento da Tecnologia Nuclear (CDTN), UFMG, Belo Horizonte-MG, Brazil. Acquisition of data, critical revision – sequence: 4 givenname: Luís Carlos Duarte surname: Ladeira fullname: Ladeira, Luís Carlos Duarte organization: Nuclear Physicist, PhD, Centro de Desenvolvimento da Tecnologia Nuclear (CDTN), UFMG, Belo Horizonte-MG, Brazil. Acquisition of data, critical revision – sequence: 5 givenname: Rafael Calvão surname: Barbuto fullname: Barbuto, Rafael Calvão organization: PhD, Assistant Professor, Medical School, UFMG, Belo Horizonte-MG, Brazil. Acquisition of data, critical revision – sequence: 6 givenname: Ivana surname: Duval-Araujo fullname: Duval-Araujo, Ivana organization: PhD, Associate Professor, Medical School, UFMG, Belo Horizonte-MG, Brazil. Critical revision, final approval |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/28538806$$D View this record in MEDLINE/PubMed |
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| References | El-Malt M (ref2) 2003; 26 Northway MG (ref5) 1988; 62 Simões Neto J (ref11) 2013; 28 Kang S (ref6) 2000; 15 Ozdemir CS (ref9) 2013; 17 Indaram AV (ref7) 2000; 95 Demir EO (ref8) 2011; 24 Kerem M (ref4) 2006; 25 Franca A (ref12) 2008; 23 Hogan NM (ref1) 2013; 50 Karliczek A (ref13) 2008; 23 Caloglu M (ref10) 2012; 8 Bedirli A (ref3) 2007; 39 Kerem M (ref14) 2006; 25 |
| References_xml | – volume: 24 start-page: 151 issue: 4 year: 2011 ident: ref8 article-title: N-acetyl-cysteine improves anastomotic wound healing after radiotherapy in rats publication-title: J Invest Surg doi: 10.3109/08941939.2011.560237 contributor: fullname: Demir EO – volume: 62 start-page: 1962 issue: 9 year: 1988 ident: ref5 article-title: Radiation proctitis in the rat Sequential changes and effects of anti-inflammatory agents publication-title: Cancer doi: 10.1002/1097-0142(19881101)62:9<1962::AID-CNCR2820620916>3.0.CO;2-T contributor: fullname: Northway MG – volume: 23 start-page: 24 issue: Suppl 1 year: 2008 ident: ref12 article-title: Effects of preoperative pelvic irradiation on colonic anastomosis healing An experimental study in rats publication-title: Acta Cir Bras doi: 10.1590/S0102-86502008000700005 contributor: fullname: Franca A – volume: 39 start-page: 141 issue: 3 year: 2007 ident: ref3 article-title: Effects of two conventional preoperative radiation schedules on anastomotic healing in the rat colon publication-title: Eur Surg Res doi: 10.1159/000100111 contributor: fullname: Bedirli A – volume: 50 start-page: 395 issue: 9 year: 2013 ident: ref1 article-title: Gastrointestinal complications of pelvic radiotherapy medical and surgical management strategies publication-title: Curr Probl Surg doi: 10.1067/j.cpsurg.2013.04.004 contributor: fullname: Hogan NM – volume: 15 start-page: 682 issue: 6 year: 2000 ident: ref6 article-title: A rat model for radiation-induced proctitis publication-title: J Korean Med Sci doi: 10.3346/jkms.2000.15.6.682 contributor: fullname: Kang S – volume: 26 start-page: e54 issue: 3 year: 2003 ident: ref2 article-title: Does the addition of glutamine to total parenteral nutrition have beneficial effect on the healing of colon anastomosis and bacterial translocation after preoperative radiotherapy publication-title: Am J Clin Oncol doi: 10.1097/01.COC.0000072505.67810.B1 contributor: fullname: El-Malt M – volume: 95 start-page: 1221 issue: 5 year: 2000 ident: ref7 article-title: Mucosal cytokine production in radiation-induced proctosigmoiditis compared with inflammatory bowel disease publication-title: Am J Gastroenterol doi: 10.1111/j.1572-0241.2000.02013.x contributor: fullname: Indaram AV – volume: 8 start-page: 260 issue: 2 year: 2012 ident: ref10 article-title: The histopathological comparison of L-carnitine with amifostine for protective efficacy on radiation-induced acute small intestinal toxicity publication-title: J Cancer Res Ther doi: 10.4103/0973-1482.98982 contributor: fullname: Caloglu M – volume: 25 start-page: 661 issue: 4 year: 2006 ident: ref4 article-title: Effects of soluble fiber on matrix metalloproteinase-2 activity and healing of colon anastomosis in rats given radiotherapy publication-title: Clin Nutr doi: 10.1016/j.clnu.2006.01.028 contributor: fullname: Kerem M – volume: 17 start-page: 1351 issue: 10 year: 2013 ident: ref9 article-title: The effect of Amifostine (Ethyol) on intestinal anastomosis in rats with radiation enteritis publication-title: Eur Rev Med Pharmacol Sci contributor: fullname: Ozdemir CS – volume: 23 start-page: 1115 issue: 11 year: 2008 ident: ref13 article-title: Preoperative irradiation with 5 x 5 Gy in a murine isolated colon loop model does not cause anastomotic weakening after colon resection publication-title: Int J Colorectal Dis doi: 10.1007/s00384-008-0507-z contributor: fullname: Karliczek A – volume: 28 start-page: 72 issue: 1 year: 2013 ident: ref11 article-title: Effects of preoperative irradiation using fractioned electron beam on the healing process of colocolonic anastomosis in rats undergoing early and late surgical intervention publication-title: Acta Cir Bras doi: 10.1590/S0102-86502013000100012 contributor: fullname: Simões Neto J – volume: 25 start-page: 661 issue: 4 year: 2006 ident: ref14 article-title: Effects of soluble fiber on matrix metalloproteinase-2 activity and healing of colon anastomosis in rats given radiotherapy publication-title: Clin Nutr doi: 10.1016/j.clnu.2006.01.028 contributor: fullname: Kerem M |
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| Snippet | To describe a new model of actinic enteritis that does not use radiotherapy machines.
Sixteen Wistar rats were divided into four groups, consisting of four... Abstract Purpose: To describe a new model of actinic enteritis that does not use radiotherapy machines. Methods: Sixteen Wistar rats were divided into four... |
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| SubjectTerms | Animals Cobalt Radioisotopes Colon - pathology Colon - radiation effects Disease Models, Animal Dose-Response Relationship, Radiation Enteritis Female Fibrosis Inflammation Radiation Radiation Injuries, Experimental - pathology Radiotherapy Rats Rats, Wistar |
| Title | Development of a new model of actinic enteritis in rats using a cobalt-60 open source and a protection device as a collimator |
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