Development of a new model of actinic enteritis in rats using a cobalt-60 open source and a protection device as a collimator

• Published in: Acta cirurgica brasileira Vol. 32; no. 4; pp. 319 - 324
• Main Authors: Nunes, Vinicius Rodrigues Taranto, Vidigal, Paula Vieira Teixeira, Pereira, Márcio Tadeu, Ladeira, Luís Carlos Duarte, Barbuto, Rafael Calvão, Duval-Araujo, Ivana
• Format: Journal Article
• Language: English
• Published: Brazil Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia 01-04-2017
• Subjects: Animals; Cobalt Radioisotopes; Colon - pathology; Colon - radiation effects; Disease Models, Animal; Dose-Response Relationship, Radiation; Enteritis; Female; Fibrosis; Inflammation; Radiation; Radiation Injuries, Experimental - pathology; Radiotherapy; Rats; Rats, Wistar
• ISSN: 0102-8650; 1678-2674; 0102-8650
• DOI: 10.1590/s0102-865020170040000007

To describe a new model of actinic enteritis that does not use radiotherapy machines.
Sixteen Wistar rats were divided into four groups, consisting of four animals each: control (group A), two weeks after irradiation (group B), five weeks after irradiation (group C) and eight weeks after irradiation (group D). Animals were given a 10Gy radiation from a Cobalt-60 natural source in a nuclear technology research center. Protections of the surrounding tissues were obtained through the usage of plumb devices with a hole in the center, which served as a collimator. We obtained irradiated and non-irradiated colons from each animal.
In group B we found an important inflammatory response in the irradiated colon, which appeared in a reduced way in group C and was minimal in group D, in which we found a relevant collagen submucosal deposition/fibrosis. In all groups, the non-irradiated colon had a lower pathological damage in comparison with the irradiated colon.
We thus described an efficient and feasible technique for obtaining an animal model of actinic enteritis.