Proteomic analysis of serum deprivation in tongue squamous cell carcinoma

• Published in: Molecular medicine reports Vol. 16; no. 6; pp. 9323 - 9330
• Main Authors: Zhang, Junfeng, Dong, Wei, Meng, Yufen, Jiang, Miao, Zhan, Zhen
• Format: Journal Article
• Language: English
• Published: Greece D.A. Spandidos 01-12-2017; Spandidos Publications; Spandidos Publications UK Ltd
• Subjects: Analysis; Apoptosis; Biomarkers; Cancer; Caspase; Caspase-7; Cell culture; Cell division; Development and progression; Experiments; Gel electrophoresis; Health aspects; Heat shock proteins; Hsp27 protein; Hsp70 protein; Kinases; Mass spectrometry; Mass spectroscopy; Oral cancer; Oxidative stress; Peroxiredoxin; Polymerase chain reaction; protein profiling; Proteins; Proteomics; Reactive oxygen species; Reverse transcription; RNA polymerase; Scientific imaging; serum deprivation; Squamous cell carcinoma; Standard deviation; Tongue; tongue squamous cell carcinoma
• ISSN: 1791-2997; 1791-3004
• DOI: 10.3892/mmr.2017.7807

The occurrence of tongue squamous cell carcinoma (TSCC) is closely correlated with serum components; however, the detailed mechanism remains to be fully elucidated. Proteomic analysis contributed to the discovery of potential biomarkers and provided an insight into TSCC at a molecular level. The present study investigated the effect of serum deprivation on the Tca-8113 TSCC cell line through protein profiling using two-dimensional gel electrophoresis and mass spectrometry, with the aim of improving TSCC diagnosis. The results showed that the Tca-8113 cells maintained proliferative capacity and resisted apoptosis following serum deprivation. A total of 43 proteins were upregulated and 45 were downregulated following serum deprivation for 24 h, compared with untreated controls (0 h). The upregulated caspase-7, heat shock protein 27 and Annexin A1, and the downregulated peroxiredoxin-6 and heat shock protein 70, were selected for verification using reverse transcription-polymerase chain reaction analysis following serum deprivation for 16 h. The results indicated that reactive oxygen species may be important in serum deprivation-induced oxidative stress.