From agro-waste to tool: biotechnological characterization and application of Ganoderma lucidum E47 laccase in dye decolorization

• Published in: 3 Biotech Vol. 9; no. 6; pp. 213 - 7
• Main Authors: Palazzolo, Martín A., Postemsky, Pablo D., Kurina-Sanz, Marcela
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01-06-2019; Springer Nature B.V
• Subjects: Acetic acid; Agricultural wastes; Agriculture; Azo dyes; Basidiomes; Biocatalysts; Bioinformatics; Biomaterials; Biotechnology; Butyl acetate; Cancer Research; Chemical industry; Chemistry; Chemistry and Materials Science; Crop residues; Decoloring; Decolorization; Dyes; Economic conditions; Ganoderma lucidum; Laccase; Mushrooms; Organic chemistry; Original; Original Article; pH effects; Selectivity; Stability; Stem Cells; Triarylmethane; Xanthene; Decolorization; Biocatalysis; Dyes; Agro-waste; Laccase; Ganoderma lucidum
• ISSN: 2190-572X; 2190-5738
• DOI: 10.1007/s13205-019-1744-2

The culture of fungal species from agro-waste allows for the sustainable preparation of valuable biotechnological products and contributes to establish the Circular Economy concept. The
Ganoderma lucidum
species is well known as producer of laccases (EC 1.10.3.2), which serves as a tool to oxidize chemicals. When producing
G. lucidum
E47 basidiomes with edible purposes out of rice crop residues, its laccase remains as by-product. In this work, we report the biotechnological characterization and application of the laccase recovered from spent cultures of the
G. lucidum
E47 strain. We detected at least one polypeptide (ca. 59 kDa) which displays attractive activity and stability values when used in the range of 18–45 °C in mildly acidic environment (pH 4.8–5.8). These parameters can be enhanced in the presence of organic cosolvents such as butyl acetate and methyl
iso
-butyl ketone, but the opposite effect is observed with solvents of lower log
P
. The best activity–stability performance is reached when the biocatalyst is used in pH 4.8 buffer with 5% (
v
/
v
) butyl acetate at 37 °C. The laccase was capable of decolorizing xanthene, azo and triarylmethane dyes, exhibiting excellent selectivity on bromocresol green and bromocresol purple. Furthermore, the biocatalyst displayed an attractive activity when assessed for the decolorization of bromocresol green in a proof-of-concept effluent biotreatment.