Recombination of Ty elements in yeast can be induced by a double-strand break

The Ty retrotransposons are the main family of dispersed repeated sequences in the yeast Saccharomyces cerevisiae. These elements are flanked by a pair of long terminal direct repeats (LTRs). Previous experiments have shown that Ty elements recombine at low frequencies, despite the fact that they ar...

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Bibliographic Details
Published in:	Genetics (Austin) Vol. 140; no. 1; pp. 67 - 77
Main Authors:	Parket, A. (Tel Aviv University, Ramat Aviv, Israel.), Inbar, O, Kupiec, M
Format:	Journal Article
Language:	English
Published:	United States Genetics Soc America 01-05-1995 Genetics Society of America
Subjects:	ADN Base Sequence Cell Cycle DNA - genetics DNA - metabolism DNA Damage DNA, Fungal - genetics DNA, Fungal - metabolism GENE Gene Conversion GENES Genetics Investigations Molecular Sequence Data NUCLEASAS NUCLEASE Polymerase Chain Reaction RECOMBINACION RECOMBINAISON Recombination, Genetic Repetitive Sequences, Nucleic Acid Retroelements - genetics SACCHAROMYCES CEREVISIAE Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - growth & development Yeast
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Summary:	The Ty retrotransposons are the main family of dispersed repeated sequences in the yeast Saccharomyces cerevisiae. These elements are flanked by a pair of long terminal direct repeats (LTRs). Previous experiments have shown that Ty elements recombine at low frequencies, despite the fact that they are present in 30 copies per genome. This frequency is not highly increased by treatments that cause DNA damage, such as UV irradiation. In this study, we show that it is possible to increase the recombination level of a genetically marked Ty by creating a double-strand break in it. This break is repaired by two competing mechanisms: one of them leaves a single LTR in place of the Ty, and the other is a gene conversion event in which the marked Ty is replaced by an ectopically located one. In a strain in which the marked Ty has only one LTR, the double-strand break is repaired by conversion. We have also measured the efficiency of repair and monitored the progression of the cells through the cell-cycle. We found that in the presence of a double-strand break in the marked Ty, a proportion of the cells is unable to resume growth
Bibliography:	9561135 F30 ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23
ISSN:	0016-6731 1943-2631 1943-2631
DOI:	10.1093/genetics/140.1.67