Expression of Cytokine and Adhesion Molecule mRNA in Atherectomy Specimens From Patients With Coronary Artery Disease

• Published in: JAPANESE CIRCULATION JOURNAL Vol. 63; no. 4; pp. 249 - 254
• Main Authors: Ishibashi, Toshiyuki, Kijima, Mikihiro, Yokoyama, Keiko, Shindo, Joji, Nagata, Kenji, Hirosaka, Akira, Techigawara, Masaaki, Abe, Yukihiko, Sato, Eiichi, Yamaguchi, Nozomi, Watanabe, Naohiko, Saito, Tomiyoshi, Maehara, Kazuhira, Ohmoto, Yasukazu, Maruyama, Yukio
• Format: Journal Article
• Language: English
• Published: Kyoto The Japanese Circulation Society 1999; Japanese Circulation Society
• Subjects: Adhesion molecules; Aged; Aged, 80 and over; Arteriosclerosis; Atherectomy, Coronary; Atherosclerosis (general aspects, experimental research); Biological and medical sciences; Blood and lymphatic vessels; Cardiology. Vascular system; Coronary artery disease; Coronary Disease - etiology; Coronary Disease - metabolism; Coronary Disease - therapy; Cytokines; Cytokines - biosynthesis; Cytokines - immunology; E-Selectin - biosynthesis; E-Selectin - immunology; Female; Humans; Intercellular Adhesion Molecule-1 - biosynthesis; Intercellular Adhesion Molecule-1 - immunology; Male; Medical sciences; Middle Aged; Polymerase Chain Reaction; RNA, Messenger - analysis; RNA, Messenger - biosynthesis; Vascular Cell Adhesion Molecule-1 - biosynthesis; Vascular Cell Adhesion Molecule-1 - immunology; Vascular disease; Human; Vascular cell adhesion molecule 1; Messenger RNA; Intercellular adhesion molecule 1; Pathogenesis; Atherosclerosis; Cytokine; Cardiovascular disease; Adhesion; Coronary heart disease
• ISSN: 0047-1828; 1347-4839
• DOI: 10.1253/jcj.63.249

Coronary arteriosclerosis is an underlying condition in acute myocardial infarction (AMI), unstable angina pectoris (UAP) and stable angina pectoris (SAP), and is also related to restenosis (RS) following coronary intervention. To investigate the pathogenesis of this condition, a quantitative reverse transcriptase polymerase chain reaction was used to determine relative levels of mRNA for interleukin (IL)-1β, IL-6, IL-8, transforming growth factor β (TGF-β), intercellular adhesion molecule (ICAM)-1, E-selectin and vascular cell adhesion molecule (VCAM)-1 using directional coronary atherectomy (DCA) specimens. Eleven patients with AMI, 7 with UAP, 10 with SAP and 6 with RS following a previous coronary intervention underwent DCA. The mRNA intensity for each molecule was expressed by comparing it with that of β-actin mRNA. The AMI and UAP patients showed high frequencies of mRNA for IL-1β, IL-8, TGF-β, and ICAM-1 together with strong intensities of expression, whereas SAP patients showed decreased mRNA expression for these molecules. Increased IL-6 mRNA expression was observed only in AMI samples. Specimens from RS patients revealed an accumulated expression of proinflammatory cytokines, except for IL-6, as well as of TGF-β. The study suggests that variation in mRNA expression may reflect the pathophysiology of specific types of coronary artery disease, and remodeling following vascular injury. (Jpn Circ J 1999; 63: 249 - 254)